A stretchable and screen-printed electrochemical sensor for glucose determination in human perspiration.
A stretchable and screen-printed electrochemical sensor for glucose determination in human perspiration.
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DOI：
10.1016/j.bios.2017.01.058
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发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Morallón E
中科院分区：
文献类型：
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作者：
Abellán-Llobregat A;Jeerapan I;Bandodkar A;Vidal L;Canals A;Wang J;Morallón E
在这里，我们提出了两种类型的全打印，高度可伸缩的，基于铂(铂)修饰石墨的廉价设备，用于生理液体中葡萄糖的测定。所述装置是：非酶传感器和酶生物传感器，后者显示出良好的结果。用固定在铂修饰的石墨上的葡萄糖氧化酶，在-0.35V(vs拟银/氯化银)下用计时电流法测定过氧化氢(H_2O_2)还原葡萄糖。该传感器可用于磷酸盐缓冲溶液(0.25M PbS，pH 7.0)中葡萄糖的测定，工作范围为33μM~0.9 mM，具有较高的灵敏度和选择性，检测下限较低。因此，它提供了一种非侵入性和体内量化人类汗液中葡萄糖水平的替代方法。该传感器已成功应用于人体汗液中的葡萄糖浓度测定，结果表明，汗液中葡萄糖浓度与商品血糖仪测得的血液中葡萄糖浓度之间存在显著的相关性。
Here we present two types of all-printable, highly stretchable, and inexpensive devices based on platinum (Pt)-decorated graphite for glucose determination in physiological fluids. Said devices are: a non-enzymatic sensor and an enzymatic biosensor, the latter showing promising results. Glucose has been quantified by measuring hydrogen peroxide (H2O2) reduction by chronoamperometry at -0.35 V (vs pseudo-Ag/AgCl) using glucose-oxidase immobilized on Pt-decorated graphite. The sensor performs well for the quantification of glucose in phosphate buffer solution (0.25 M PBS, pH 7.0), with a working range between 33 μM and 0.9 mM, high sensitivity and selectivity, and a low limit of detection (LOD). Thus it provides an alternative non-invasive and on-body quantification of glucose levels in human perspiration. This biosensor has been successfully applied on real human perspiration samples and results also show a significant correlation between glucose concentration in perspiration and glucose concentration in blood measured by a commercial glucose meter.