Pulse-modulated photoacoustic measurements reveal strong gas-uptake component at high CO2-concentrations
Pulse-modulated photoacoustic measurements reveal strong gas-uptake component at high CO2-concentrations
复制标题
脉冲调制光声测量揭示了高二氧化碳浓度下强烈的气体吸收成分
DOI：
10.1007/bf00035539
复制
发表时间：
1992
影响因子：
3.7
通讯作者：
U. Schreiber
中科院分区：
文献类型：
--
作者：
H. Reising;U. Schreiber
本文研究了高CO_2浓度对烟叶光声信号的影响，采用了一种新的脉冲调制方法，在毫秒时域内同时提供光热分量和光压分量的信息。发现高CO2浓度诱导大的气体摄取信号。叶绿素荧光的同时测量表明，吸收信号与能量依赖的荧光猝灭。非常相似的CO2-浓度依赖性被发现在甲基紫精，这是已知的催化O2-还原的存在下，并在甘油醛的存在下，这块卡尔文循环和光呼吸。有人建议，CO2增强的摄取信号可能反映在Mehler反应中的O2摄取。然而，不排除也涉及由光诱导的基质碱化引起的快速CO2增溶或CO2结合。当封闭光声腔中的CO2-浓度由于暗呼吸而增加时，也诱导强摄取。这些发现对于解释光声数据的结果（例如，“低光效应”）和O2依赖的电子流的调节作用进行了讨论。
The effect of high CO2-concentration on photoacoustic signals from tobacco leaves is studied by means of a recently developed pulse modulation method which provides simultaneous information on photothermal and photobaric components in the millisecond time domain. High CO2-concentrations are found to induce large gas-uptake signals. Simultaneous measurements of chlorophyll fluorescence suggest that the uptake signals are correlated with energy-dependent fluorescence quenching. Very similar CO2-concentration dependencies are found in the absence and presence of methylviologen, which is known to catalyze O2-reduction, and in the presence of glyceraldehyde, which blocks Calvin cycle and photorespiration. It is suggested that the CO2-enhanced uptake signal is likely to reflect O2-uptake in the Mehler reaction. However, it is not ruled out that also rapid CO2-solubilisation or CO2-binding caused by light-induced stroma alkalisation are involved. Strong uptake is also induced when the CO2-concentration in the closed photoacoustic chamber increases due to dark-respiration. The consequences of these findings with respect to the interpretation of photoacoustic data (e.g., ‘low-light effect’) and to the regulatory role of O2-dependent electron flow are discussed.
影响因子：
7.4
作者：
DEMMIG, B;WINTER, K;CZYGAN, FC
通讯作者：
CZYGAN, FC