Calpain-5 gene expression in the mouse eye and brain.
Calpain-5 gene expression in the mouse eye and brain.
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DOI：
10.1186/s13104-017-2927-8
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发表时间：
2017-11-21
影响因子：
1.8
通讯作者：
Mahajan VB
中科院分区：
文献类型：
--
作者：
Schaefer K;Mahajan M;Gore A;Tsang SH;Bassuk AG;Mahajan VB
我们的目的是表征CAPN 5基因在小鼠中枢神经系统中的表达。用两种设计用于结合CAPN 5 RNA的高亲和力RNA寡核苷酸类似物和一种乱序对照寡核苷酸探测小鼠脑和眼切片。图像在明视野下拍摄。 在小鼠乳腺癌肿瘤组织上验证CAPN 5 RNA探针。在眼睛中，CAPN 5在视网膜的神经节细胞、内核层和外核层中表达。睫状体或虹膜中由于黑色素密度高而不能检测到信号。在脑中，CAPN 5在海马和小脑的颗粒细胞层中表达。脑桥有散在表达。视觉皮层显示出微弱的信号。大脑中的大多数信号是点状的。
Our objective was to characterize CAPN5 gene expression in the mouse central nervous system. Mouse brain and eye sections were probed with two high-affinity RNA oligonucleotide analogs designed to bind CAPN5 RNA and one scramble, control oligonucleotide. Images were captured in brightfield. CAPN5 RNA probes were validated on mouse breast cancer tumor tissue. In the eye, CAPN5 was expressed in the ganglion cell, inner nuclear and outer nuclear layers of the retina. Signal could not be detected in the ciliary body or the iris because of the high density of melanin. In the brain, CAPN5 was expressed in the granule cell layers of the hippocampus and cerebellum. There was scattered expression in pons. The visual cortex showed faint signal. Most signal in the brain was in a punctate pattern.