Intracerebroventricular injection of ghrelin decreases wheel running activity in rats.
Intracerebroventricular injection of ghrelin decreases wheel running activity in rats.
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脑室内注射生长素释放肽可降低大鼠的轮跑活动。
DOI：
10.1016/j.peptides.2016.11.005
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Sakaue H.
中科院分区：
文献类型：
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作者：
Miyatake Y;Shiuchi T;Mawatari K;Toda S;Taniguchi Y;Futami A;Sato F;Kuroda M;Sebe M;Tsutsumi R;Harada N;Minokoshi Y;Kitamura T;Gotoh K;Ueno M;Nakaya Y;Sakaue H.
有越来越多的兴趣，阐明自愿行使调节的分子机制。在这项研究中，我们研究了中枢神经系统如何调节运动。我们使用SPORTS大鼠，这是建立在我们的实验室作为一个高度自愿的小鼠运动模型。SPORTS大鼠显示较低水平的血清ghrelin相比，Wistar大鼠的父母线。侧脑室和腹腔注射ghrelin可降低SPORTS大鼠的跑轮活动。此外，每天向Wistar大鼠的侧脑室注射生长激素释放肽抑制剂JMV 3002增加了车轮运行活动。共同管理的obestatin抑制生长激素释放肽诱导的食物摄入量的增加，但没有抑制生长激素释放肽诱导的抑制自愿运动大鼠。运动组大鼠下丘脑和海马的生长激素促分泌素受体（GHSR）与对照组无明显差异。本研究通过对新生的SPORTS大鼠给予谷氨酸（MSG）建立了弓状核毁损模型.向味精处理的大鼠注射ghrelin减少了自愿运动，但没有增加食物摄入，这表明轮运行活动不是由调节进食的弓状核神经元控制的。这些结果提供了新的见解的机制，ghrelin调节自主活动独立的弓状核神经元。
There is an increasing interest in elucidating the molecular mechanisms by which voluntary exercise is regulated. In this study, we examined how the central nervous system regulates exercise. We used SPORTS rats, which were established in our laboratory as a highly voluntary murine exercise model. SPORTS rats showed lower levels of serum ghrelin compared with those of the parental line of Wistar rats. Intrac-erebroventricular and intraperitoneal injection of ghrelin decreased wheel-running activity in SPORTS rats. In addition, daily injection of the ghrelin inhibitor JMV3002 into the lateral ventricles of Wistar rats increased wheel-running activity. Co-administration of obestatin inhibited ghrelin-induced increases in food intake but did not inhibit ghrelin-induced suppression of voluntary exercise in rats. Growth hormone secretagogue receptor (GHSR) in the hypothalamus and hippocampus of SPORTS rats was not difference that in control rats. We created an arcuate nucleus destruction model by administering monosodium glutamate (MSG) to neonatal SPORTS rats. Injection of ghrelin into MSG-treated rats decreased voluntary exercise but did not increase food intake, suggesting that wheel-running activity is not controlled by the arcuate nucleus neurons that regulate feeding. These results provide new insights into the mechanism by which ghrelin regulates voluntary activity independent of arcuate nucleus neurons.