Exciton Plasmon Interaction between AuNPs/Graphene Nanohybrids and CdS Quantum Dots/TiO2 for Photoelectrochemical Aptasensing of Prostate-Specific Antigen
Exciton Plasmon Interaction between AuNPs/Graphene Nanohybrids and CdS Quantum Dots/TiO2 for Photoelectrochemical Aptasensing of Prostate-Specific Antigen
复制标题
AuNPs/石墨烯纳米杂化物和 CdS 量子点/TiO2 之间的激子等离子体相互作用用于前列腺特异性抗原的光电化学适体传感
DOI：
10.1021/acssensors.7b00899
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发表时间：
2018-03-01
期刊：
影响因子：
8.9
通讯作者：
Tang, Dianping
中科院分区：
文献类型：
--
作者：
Cai, Guoneng;Yu, Zhengzhong;Tang, Dianping
设计了一种基于靶标诱导金纳米粒子包覆石墨烯纳米片(AuNPs/GN)从CdS量子点功能化介孔二氧化钛(CDS QDS/Ti02)中解离出来的竞争置换反应策略，通过激子-等离子体相互作用(EPI)实现对前列腺特异性抗原(PSA)的灵敏光电化学(PEC)传感。为了构建这样的适配系统，首先将捕获DNA共价连接到修饰电极上，然后通过部分碱基与捕获DNA的杂交链反应将AuNPs/GN标记的PSA适配子连接到生物功能化的CDS量子点/二氧化钛上。由于能量转移，AuNPs/GN的引入有效地猝灭了CDS量子点/TiO2光电流。当添加目标PSA时，夹在CDS量子点/TiO2和AuNPs/GN之间的适配子与被分析物反应，从而导致AuNPs/GN从CDS QDS/TiO2中解离出来，从而增加了光电流。在最佳条件下，该平台对PSA的检测具有较高的灵敏度，检测的线性范围为1.0~8.0 ng/mL，检测下限为0.52 pg/mL。研究了该体系中激子-等离子体相互作用的粒子间距和与EPI效应对应的AuNPs的含量。用于目标PSA的分析具有良好的选择性和较高的重现性。重要的是，对PEC适配子传感器用于人血清标本和新生儿小牛血清稀释PSA标准品的测定的准确性和基质效应进行了评估，给出了与参考PSA酶联免疫吸附试剂盒很好的匹配结果。
A competitive-displacement reaction strategy based on target-induced dissociation of gold nanoparticle coated graphene nanosheet (AuNPs/GN) from CdS quantum dot functionalized mesoporous titanium dioxide (CdS QDs/TiO2) was designed for the sensitive photoelectrochemical (PEC) aptasensing of prostate-specific antigen (PSA) through the exciton-plasmon interaction (EPI) between CdS QDs and AuNPs. To construct such an aptasensing system, capture DNA was initially conjugated covalently onto CdS QDs/TiO2-modified electrode, and then AuNPs/GN-labeled PSA aptamer was bound onto biofunctionalized CdS QDs/TiO2 via hybridization chain reaction of partial bases with capture DNA. Introduction of AuNPs/GN efficiently quenched the photocurrent of CdS QDs/TiO2 thanks to energy transfer. Upon addition of target PSA, the sandwiched aptamer between CdS QDs/TiO2 and AuNPs/GN reacted with the analyte analyte, thus resulting in the dissociation of AuNPs/GN from the CdS QDs/TiO2 to increase the photocurrent. Under optimum conditions, the aptasensing platform exhibited a high sensitivity for PSA detection within a dynamic linear range of 1.0 pg/mL to 8.0 ng/mL at a low limitat of detection of 0.52 pg/mL. The interparticle distance of exciton-plasmon interaction and contents of AuNPs corresponding to EPI effect in this system were also studied. Good selectivity and high reproducibility were obtained for the analysis of target PSA. Importantly, the accuracy and matrix effect of PEC aptasensor was evaluated for the determination of human serum specimens and newborn calf serum-diluted PSA standards, giving a well-matched result with the referenced PSA ELISA kit.