Differences in lipid accumulation and mobilization in the hepatopancreas and ovary of female mud crab (Scylla paramamosain, Estampador, 1949) during ovarian development
Differences in lipid accumulation and mobilization in the hepatopancreas and ovary of female mud crab (Scylla paramamosain, Estampador, 1949) during ovarian development
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DOI：
10.1016/j.aquaculture.2022.739046
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发表时间：
2022-11-22
期刊：
影响因子：
4.5
通讯作者：
Wen，Xiaobo
中科院分区：
文献类型：
--
作者：
Chen，Baojia;Zheng，Jia;Wen，Xiaobo
众所周知，在卵巢发育过程中，亲蟹的卵巢和肝胰腺之间脂质和脂肪酸的积累和动员存在很大差异。然而，其根本机制尚不完全清楚。本研究分析比较了拟青蟹各成熟阶段脂质代谢相关基因的生化组成和表达谱。结果表明，在卵巢发育过程中，卵巢沉积了大量的总脂质，其中以中性脂质为主，主要是三酰甘油（TAG），富含高水平的饱和脂肪酸。肝胰腺中微粒体甘油三酯转移蛋白 (MTP)、超量位点蛋白 4 (SURF4) 和载脂甲壳素 (ApoCr) 转录水平显着增加，卵巢发育过程中卵巢中卵黄蛋白原受体 (VTGR) 的转录水平也显着增加 (P< 0.05)。我们推测了可能的机制是MTP将TAG转运至ApoCr，然后SURF4在卵巢发育过程中将ApoCr从肝胰腺转运至血淋巴，最后ApoCr被卵母细胞的VTGR吸收，导致更高的性腺体指数（GSI）和受脂肪生成和脂肪分解基因调控的脂质沉积。这些结果被认为为进一步雌性泥蟹生殖生物学研究提供了基础信息。
The accumulation and mobilization of lipids and fatty acids are known to vary greatly between the ovary and hepatopancreas of crab broodstock during ovarian development. However, the underlying mechanism is not completely understood. In the present study, we analyzed and compared the biochemical compositions and expression profiles of lipid metabolism-related genes during each maturation stage inScylla paramamosain. The results revealed that ovaries deposited large amounts of total lipid contents with a predominance of neutral lipids, mainly triacylglycerol (TAG), rich in high levels of saturated fatty acids throughout ovarian development. Microsomal triglyceride transfer protein (MTP), surfeit locus protein 4 (SURF4) and apolipocrustacein (ApoCr) transcription levels were significantly increased in the hepatopancreas as did transcription levels of the vitellogenin receptor (VTGR) in the ovary during ovarian development (P< 0.05). We speculated the possible mechanism by which MTP transported TAG to ApoCr, then SURF4 conveyed ApoCr from the hepatopancreas to the hemolymph during ovarian development, and finally, ApoCr was absorbed by the VTGR of oocytes, leading to a higher gonadosomatic index (GSI) and lipid deposition regulated by lipogenesis and lipolysis genes. These results are considered to provide fundamental information for further reproductive biology research on female mud crabs.