In vivo evidence for a dependence on interleukin 15 for survival of natural killer cells
In vivo evidence for a dependence on interleukin 15 for survival of natural killer cells
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DOI：
10.1182/blood-2001-12-0293
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发表时间：
2002-11-15
期刊：
影响因子：
20.3
通讯作者：
Caligiuri, MA
中科院分区：
文献类型：
--
作者：
Cooper, MA;Bush, JE;Caligiuri, MA
细胞内稳态需要细胞生成、细胞存活和细胞死亡之间的平衡。骨髓前体细胞产生自然杀伤细胞（NK）需要白细胞介素15 (IL-15)；然而，对于体内控制成熟NK细胞存活的因素所知甚少。由于缺乏IL-15的小鼠（IL-15（-/-）小鼠）容易发育NK细胞，成熟NK细胞能否在缺乏IL-15的环境中存活尚不清楚。我们假设成熟NK细胞的存活可能确实需要IL-15。在体内，用5-（和6）-羧基荧光素二乙酸琥珀酰酯（CFSE）标记的新分离的NK细胞被过性地转移到IL-15（-/-）小鼠和同巢对照（IL-15(+/-)）小鼠中。转染后36小时内，IL-15（-/-）和IL-15（+/-）小鼠均检测到NK细胞；然而，显著高于(P
Cellular homeostasis requires a balance between cell production, cell survival, and cell death. Production of natural killer (NK) cells from bone marrow precursor cells requires Interleukin 15 (IL-15); however, very little is known about the factors controlling survival of mature NK cells in vivo. Because mice deficient in IL-15 (IL-15(-/-) mice) fall to develop NK cells, it is not known whether mature NK cells can survive in an environment lacking IL-15. We hypothesized that IL-15 might indeed be required for survival of mature NK cells In vivo, Freshly isolated NK cells labeled with 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) were adoptively transferred into IL-15(-/-) mice and littermate control (IL-15(+/-)) mice. Within 36 hours after transfer, NK cells were detected in both IL-15(-/-) and IL-15(+/-) mice; however, significantly more (P