Anti-anxiety effects of Apocynum venetum L. in the elevated plus maze test
Anti-anxiety effects of Apocynum venetum L. in the elevated plus maze test
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DOI：
10.1016/j.jep.2006.09.035
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发表时间：
2007-04-04
影响因子：
5.4
通讯作者：
Butterweck, Veronika
中科院分区：
文献类型：
--
作者：
Grundmann, Oliver;Nakajima, Jun-Ichiro;Butterweck, Veronika
本研究的目的是使用小鼠高架十字迷宫（高架十字迷宫）表征从罗布麻叶（AV）制备的乙醇提取物的推定抗焦虑样活性。雄性C75 BL/6小鼠在行为评价前1 h分别经口给予AV提取物或阳性对照地西泮和丁螺环酮。AV提取物的单次处理显著增加了30和125 mg/kg p.o.剂量的曲马多的开放臂上花费的时间百分比和进入开放臂的次数，分别该作用与苯二氮卓类地西泮（1.5 mg/kg p.o.）和5-HT 1A激动剂丁螺环酮（10 mg/kg p.o.）。苯二氮卓类拮抗剂氟马西尼（0.3mg/kg）可有效地拮抗AV（125 mg/kg）的作用。然而，AV提取物的作用只能部分被非特异性5-HT 1A受体拮抗剂WAY-100635（0.5 mg/kg i. p.）阻断。在旷场试验中，地西泮和丁螺环酮以及AV浸提液均未产生任何明显的行为变化或运动功能障碍。这些结果表明，AV提取物是一种有效的抗焦虑剂，并表明这种植物的抗焦虑活性主要是通过GABA能系统介导的。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
The purpose of this study was to characterize the putative anxiolytic-like activity of an ethanolic extract prepared from the leaves of Apocynum venetum (AV) using the elevated plus maze (EPM) in mice. Male C75BL/6 mice were either treated orally with the AV extract or the positive controls diazepam and buspirone, respectively, 1 h before behavioral evaluation in the EPM. A single treatment of AV extract markedly increased the percentage time spent on and the number of entries into the open arms of the EPM in doses of 30 and 125 mg/kg p.o., respectively. This effect was comparable to that of the benzodiazepine diazepam (1.5 mg/kg p.o.) and the 5-HT1A agonist buspirone (10 mg/kg p.o.). The effects of AV in 125 mg/kg were effectively antagonized by the benzodiazepine antagonist flumazenil (0.3 mg/kg i.p.). However, the effects of AV extract could only partially be blocked by the unspecific 5-HT1A receptor antagonist WAY-100635 (0.5 mg/kg i.p.). Neither diazepam and buspirone nor the AV extract produced any overt behavioral change or motor dysfunction in the open field test. These results indicate that AV extract is an effective anxiolytic agent, and suggest that the anxiolytic-like activities of this plant are mainly mediated via the GABAergic system. (c) 2006 Elsevier Ireland Ltd. All rights reserved.