Neisseria conserved protein DMP19 is a DNA mimic protein that prevents DNA binding to a hypothetical nitrogen-response transcription factor.
Neisseria conserved protein DMP19 is a DNA mimic protein that prevents DNA binding to a hypothetical nitrogen-response transcription factor.
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DOI：
10.1093/nar/gks177
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发表时间：
2012-07
影响因子：
14.9
通讯作者：
Wang AH
中科院分区：
文献类型：
--
作者：
Wang HC;Ko TP;Wu ML;Ku SC;Wu HJ;Wang AH
DNA模拟蛋白占据DNA结合蛋白的DNA结合位点，并阻止这些位点被DNA接近。我们在这里表明，奈瑟氏球菌保守的假设蛋白质DMP 19作为一个DNA模拟。DMP 19的晶体结构在表面上显示出类似dsDNA的负电荷分布，这表明该蛋白应该被添加到已知的DNA模拟蛋白的短名单中。另一种相关蛋白质NHTF（奈瑟氏菌假设转录因子）的晶体结构提供了证据，表明它是转录因子的外源性反应元件（XRE）家族的成员。NHTF与含有5′-TGTNAN 11 TNACA-3′识别盒的回文DNA序列结合，该识别盒控制NHTF相关操纵子的表达，其中保守的氮反应蛋白[即（蛋白质-PII）尿苷酰转移酶]被编码。DMP 19和NHTF之间的互补表面电荷表明特定的电荷-电荷相互作用。在DNA结合试验中，我们发现DMP 19可以阻止NHTF与其DNA结合位点的结合。最后，我们使用原位基因调控试验来提供证据，证明NHTF是其下游基因的阻遏物，并且DMP 19可以中和这种作用。因此，我们得出结论，DMP 19和NHTF的相互作用提供了一种新的基因调控机制，在奈瑟氏菌。
DNA mimic proteins occupy the DNA binding sites of DNA-binding proteins, and prevent these sites from being accessed by DNA. We show here that the Neisseria conserved hypothetical protein DMP19 acts as a DNA mimic. The crystal structure of DMP19 shows a dsDNA-like negative charge distribution on the surface, suggesting that this protein should be added to the short list of known DNA mimic proteins. The crystal structure of another related protein, NHTF (Neisseria hypothetical transcription factor), provides evidence that it is a member of the xenobiotic-response element (XRE) family of transcriptional factors. NHTF binds to a palindromic DNA sequence containing a 5′-TGTNAN11TNACA-3′ recognition box that controls the expression of an NHTF-related operon in which the conserved nitrogen-response protein [i.e. (Protein-PII) uridylyltransferase] is encoded. The complementary surface charges between DMP19 and NHTF suggest specific charge–charge interaction. In a DNA-binding assay, we found that DMP19 can prevent NHTF from binding to its DNA-binding sites. Finally, we used an in situ gene regulation assay to provide evidence that NHTF is a repressor of its down-stream genes and that DMP19 can neutralize this effect. We therefore conclude that the interaction of DMP19 and NHTF provides a novel gene regulation mechanism in Neisseria spps.
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影响因子：
6.7
作者：
Aspholm M;Aas FE;Harrison OB;Quinn D;Vik A;Viburiene R;Tønjum T;Moir J;Maiden MC;Koomey M
通讯作者：
Koomey M
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
14.9
作者：
McMahon SA;Roberts GA;Johnson KA;Cooper LP;Liu H;White JH;Carter LG;Sanghvi B;Oke M;Walkinshaw MD;Blakely GW;Naismith JH;Dryden DT
通讯作者：
Dryden DT
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL
影响因子：
8
作者：
Parsons, LM;Liu, F;Orban, J
通讯作者：
Orban, J