NEOVASCULARIZATION AND THE APPEARANCE OF MORPHOLOGICAL-CHARACTERISTICS OF THE BLOOD-BRAIN-BARRIER IN THE EMBRYONIC MOUSE CENTRAL-NERVOUS-SYSTEM
NEOVASCULARIZATION AND THE APPEARANCE OF MORPHOLOGICAL-CHARACTERISTICS OF THE BLOOD-BRAIN-BARRIER IN THE EMBRYONIC MOUSE CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1016/0165-3806(93)90031-5
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发表时间：
1993-10-15
期刊：
影响因子：
--
通讯作者：
STEINER, M
中科院分区：
文献类型：
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作者：
BAUER, HC;BAUER, H;STEINER, M
用超微结构分析方法研究了小鼠中枢神经系统（CNS）胚胎发育过程中血管化及血脑屏障（BBB）相关形态学特征的表达。在胚胎第9天（E9）毛细血管只发现在神经周间充质组织。这些毛细血管有穿孔，发现周细胞样细胞（PC）与血管壁相连。E10前后，内皮细胞（EC）开始与PC一起侵入神经内切片。在这个阶段，迁移的内皮细胞失去了他们的开窗，并表现出大量的，部分延长的连接复合体，出现在一些地方的“紧”。在E10时观察到第一个神经内吻合丛，所有毛细血管腔内均存在血细胞。虽然连接复合物的数量保持不变，在神经内毛细血管，吞饮小泡的频率显着下降，从E10到E17。这些发现表明，从神经内血管形成的第一天起，血脑屏障的形态特征就存在于早期胚胎小鼠大脑皮层中。
Vascularization and expression of blood-brain barrier (bbb)-associated morphological characteristics during the embryonic development of the mouse cnetral nervous system (CNS) was studied by ultrastructural analysis. At embryonic day 9 (E9) capillaries were only found in the perineural mesenchymal tissue. These capillaries showed fenestrations, and pericyte like cells (PC) were found joined to the vessel walls. Around E10 endothelial cells (EC) together with PC started invading the intraneural section. At this stage, the immigrating endothelial cells lost their fenestrations and exhibited numerous, partly extended junctional complexes, which appeared 'tight' in some places. A first intraneural anastomotic plexus was observed at E10, as evidenced by the presence of blood cells in all capillary lumens. While the number of junctional complexes remained constant in intraneural capillaries, the frequency of pinocytotic vesicles decreased significantly from E10 to E17. These findings indicate that from the first day of intraneural vascularization onwards, the morphological properties of the bbb are present in the early embryonic mouse cerebral cortex.