Protective effect of unilateral and bilateral ear plugs on noise-induced hearing loss: Functional and morphological evaluation in animal model
Protective effect of unilateral and bilateral ear plugs on noise-induced hearing loss: Functional and morphological evaluation in animal model
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DOI：
10.4103/1463-1741.134915
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发表时间：
2014-05-01
期刊：
影响因子：
0.7
通讯作者：
Park, Shi-Nae
中科院分区：
文献类型：
--
作者：
Kim, Dong-Kee;Park, Yooyeon;Park, Shi-Nae
本研究旨在从形态学和功能学两方面评价耳塞对噪声的即刻防护效果。1月龄的29只雄性C57 BL/6小鼠。将受试者分为正常对照组（G1）、双侧堵塞组（G2）、单侧堵塞组（G3）和噪声对照组（G4），后3组暴露于110声压级的白色噪声60 min。噪声暴露后G2和G3塞耳听力无明显变化，而G3和G4未塞耳听力明显下降。在形态学上，G3和G4未堵塞耳Corti器明显退化，传出神经钮的平均数目和直径明显减少。G3代的堵塞穗在形态学上也表现出轻微的变化。噪声暴露后，在传出终末或耳蜗提取物中观察到a-突触核蛋白的减少。在噪声性听力损失动物模型上，耳塞对噪声暴露的保护作用从形态学和功能学上得到了证实。更确切的作用机制有待于从细胞或超微结构水平进一步研究。
The aim of the following study is to evaluate immediate protective effect of ear plug from noise morphologically and functionally. An 1-month aged 29 male C57BL/6 mice. Subjects were divided into four groups as normal control(G1), bilaterally plugged group (G2), unilaterally plugged group (G3) and noise control group (G4) and later 3 groups were exposed to 110 sound pressure level white noise for 60 min. Immediately after noise exposure, audiologic tests were performed and cochlear morphology and expression levels of a-synuclein in the cochlea were investigated. There were no functional changes in G2 and plugged ears of G3 after noise exposure, whereas unplugged ears of G3 and G4 showed significant hearing loss. In morphological study, there were a significant degeneration of the organ of Corti and mean number and diameter of efferent buttons, in unplugged ears of G3 and G4. Plugged ears of G3 also showed mild changes in morphological study. Reduction of a-synuclein was observed at the efferent terminals or cochlear extracts after noise exposure. The protective effect of ear plug on noise exposure was proven morphologically and functionally in the animal model of noise-induced hearing loss. Further study on cellular or ultrastructural level with ear plug will be needed to reveal more precise mechanism.