Surface modification using silanated poly(ethylene glycol)s
Surface modification using silanated poly(ethylene glycol)s
复制标题
DOI：
10.1016/s0142-9612(99)00224-0
复制
发表时间：
2000-03-01
期刊：
影响因子：
14
通讯作者：
Park, K
中科院分区：
文献类型：
--
作者：
Jo, S;Park, K
已知表面接枝的聚乙二醇（PEG）分子可以防止蛋白质吸附到表面。聚乙二醇分子的蛋白排斥特性通过共价接枝得到最大化。我们合成了硅烷化单甲氧基聚乙二醇（m-PEG），将聚乙二醇共价接枝到具有氧化层的表面。合成了两种不同的三烷氧基化聚乙二醇并对其进行了表征。将m-PEG与3-异氰酸异丙基三乙氧基硅烷通过聚氨酯键直接偶联制备了首个三烷氧基硅化聚乙二醇（硅烷化聚乙二醇I）。在与3-巯基丙基三甲氧基硅烷发生硅化反应之前，m-PEG依次与1,6-二异氰酸酯己烷和10 -十一烯-1-醇反应，制备了另一种硅化PEG（硅化PEG II），其疏水结构域位于PEG和硅烷结构域之间。将硅化的聚乙二醇I和聚乙二醇II接枝到玻璃上，这是我们研究中使用的模型表面。通过接触角、x射线光电子能谱（XPS）和原子力显微镜（AFM）对聚乙二醇接枝玻璃表面进行了表征。尽管接触角随着聚乙二醇体积浓度从0.1 mg/ml增加到20 mg/ml而变化不大，但XPS测量的表面原子浓度表明聚乙二醇接枝成功。表面聚乙二醇接枝增加了表面碳的浓度，但降低了表面硅的浓度。高分辨率Cls光谱显示，与对照表面相比，peg接枝表面的醚碳含量较高，碳氢化合物组成较低。AFM图像显示，随着接枝体积浓度的增加，更多的PEG分子接枝到表面。干燥表面的AFM图像显示，表面没有完全被PEG分子覆盖。然而，水化后，表面似乎被完全覆盖，这可能是由于接枝PEG链的水化作用。与对照表面相比，硅烷化聚乙二醇修饰的玻璃表面减少了95%以上的纤维蛋白原吸附。硅化聚乙二醇为聚乙二醇接枝到含有氧化层的表面提供了一种简单的方法。(C) 2000 Elsevier Science Ltd.版权所有。
Surface-grafted poly(ethylene glycol) (PEG) molecules are known to prevent protein adsorption to the surface. The protein-repulsive property of PEG molecules are maximized by covalent grafting. We have synthesized silanated monomethoxy-PEG (m-PEG) for covalent grafting of PEG to surfaces with oxide layers. Two different trialkoxysilylated PEGs were synthesized and characterized. The first trialkoxysilylated PEG was prepared by direct coupling of m-PEG with 3-isocyanatopropyltriethoxysilane through a urethane bond (silanated PEG I). The other silanated PEG (silanated PEG II) containing a long hydrophobic domain between PEG and a silane domain was prepared by reacting m-PEG with 1,6-diisocyanatohexane and I0-undecen-1-ol in sequence before silylation with 3-mercaptopropyl trimethoxysilane. Silanated PEGs I and II were grafted onto glass, a model surface used in our study. The PEG-grafted glass surfaces were characterized by contact angle, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Although contact angle did not change much as the bulk concentration of silanated PEG used for grafting increased from 0.1 to 20 mg/ml for both PEGs I and II, the surface atomic concentrations from XPS measurements showed successful PEG grafting. Surface PEG grafting increased concentration of surface carbon but decreased silicone concentration. The high resolution Cls spectra showed higher ether carbon with lower hydrocarbon compositions for the PEG-grafted surfaces compared to the control surface. AFM images showed that more PEG molecules were grafted onto the surface as the bulk concentration used for grafting was increased. AFM images of the dried surfaces showed that the surfaces were not completely covered by PEG molecules. After hydration, however, the surface appears to be covered completely probably due to the hydration of the grafted PEG chains. Glass surfaces modified with silanated PEGs reduced fibrinogen adsorption by more than 95% as compared with the control surface. Silanated PEGs provides a simple method for PEG grafting to the surface containing oxide layers. (C) 2000 Elsevier Science Ltd. All rights reserved.