ProPhylER: A curated online resource for protein function and structure based on evolutionary constraint analyses
ProPhylER: A curated online resource for protein function and structure based on evolutionary constraint analyses
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DOI：
10.1101/gr.097121.109
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发表时间：
2010-01-01
期刊：
影响因子：
7
通讯作者：
Sidow, Arend
中科院分区：
文献类型：
--
作者：
Binkley, Jonathan;Karra, Kalpana;Sidow, Arend
ProPhylER（Protein Phylogeny and Evolutionary Rates）是一个新一代的蛋白质组资源，它使用比较序列分析来预测真核蛋白质的约束和突变影响。其目的是通过进化约束分析的预测能力，为任何与蛋白质功能和结构相关的研究项目提供信息。ProPhylER目前拥有近9000个相关蛋白质簇，包括超过20万个序列。它通过两个接口提供数据。“ProPhylER界面”显示序列空间中的预测分析;“CrystalPainter”将进化约束映射到解决的蛋白质结构上。在这里，我们总结了ProPhylER的数据内容和分析管道，演示了ProPhylER的接口的使用，并评估ProPhylER独特的区域分析的进化约束。ProPhylER区域分析的高准确性补充了其单位点分析的高分辨率，以有效地指导和告知结构-功能研究并预测多态性的影响。
ProPhylER (Protein Phylogeny and Evolutionary Rates) is a next-generation curated proteome resource that uses comparative sequence analysis to predict constraint and mutation impact for eukaryotic proteins. Its purpose is to inform any research program for which protein function and structure are relevant, by the predictive power of evolutionary constraint analyses. ProPhylER currently has nearly 9000 clusters of related proteins, including more than 200,000 sequences. It serves data via two interfaces. The "ProPhylER Interface" displays predictive analyses in sequence space; the "CrystalPainter" maps evolutionary constraints onto solved protein structures. Here we summarize ProPhylER's data content and analysis pipeline, demonstrate the use of ProPhylER's interfaces, and evaluate ProPhylER's unique regional analysis of evolutionary constraint. The high accuracy of ProPhylER's regional analysis complements the high resolution of its single-site analysis to effectively guide and inform structure-function investigations and predict the impact of polymorphisms.