Hydrophobic displacement chromatography of proteins
Hydrophobic displacement chromatography of proteins
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DOI：
10.1002/(sici)1097-0290(20000620)68:6
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发表时间：
2000-06-20
影响因子：
3.8
通讯作者：
Cramer, SM
中科院分区：
文献类型：
--
作者：
Shukla, AA;Sunasara, KM;Cramer, SM
在疏水作用和反相色谱系统中，使用低分子量置换剂成功地进行了蛋白质的置换色谱。用于疏水置换色谱的置换剂是含有几个短烷基和/或芳基的水溶性带电分子。采用光谱学来验证在这些疏水支持物上置换的蛋白质没有结构变化。采用反相材料上的置换色谱法从其密切相关的变体中纯化生长因子蛋白，证明了通过疏水置换色谱法可以实现的高分辨率。该过程将置换色谱的高分辨率/高通量特性与这些疏水载体的独特选择性相结合，为色谱工程师提供了蛋白质制备纯化的强大工具。(C)John Wiley & Sons，Inc.
Displacement chromatography of proteins was successfully carried out in both hydrophobic interaction and reversed-phase chromatographic systems using low-molecular weight displacers. The displacers employed for hydrophobic displacement chromatography were water soluble, charged molecules containing several short alkyl and/or aryl groups. Spectroscopy was employed to verify the absence of structural changes to the proteins displaced on these hydrophobic supports. Displacement chromatography on a reversed-phase material was employed to purify a growth factor protein from its closely related variants, demonstrating the high resolutions that can be achieved by hydrophobic displacement chromatography. This process combines the high-resolution/high-throughput characteristics of displacement chromatography with the unique selectivity of these hydrophobic supports and offers the chromatographic engineer a powerful tool for the preparative purification of proteins. (C) 2000 John Wiley & Sons, Inc.