Five histidine kinases perceive osmotic stress and regulate distinct sets of genes in Synechocystis
Five histidine kinases perceive osmotic stress and regulate distinct sets of genes in Synechocystis
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DOI：
10.1074/jbc.m410162200
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发表时间：
2004-12-17
影响因子：
4.8
通讯作者：
Murata, N
中科院分区：
文献类型：
--
作者：
Paithoonrangsarid, K;Shumskaya, MA;Murata, N
微生物通过大量基因表达水平的变化对高渗胁迫作出反应。这种反应对于适应新的渗透环境是必不可少的。为了确定参与高渗胁迫引起的信号的感知和转导的因素，我们研究了集胞藻属PCC 6803的反应，这已被证明是一种特别有用的微生物在类似的分析。通过基因芯片和狭缝杂交技术筛选集胞藻中组氨酸激酶（Hiks）和反应调节因子（Rres）的敲除文库，并鉴定了几个Hik-Rre系统，分别命名为Hik 33-Rre 31、Hik 34-Rre 1、Hik 10-Rre 3和Hik 16-Hik 41-Rre 17。我们还确定了Hik 2-Rre 1作为一个假定的额外的双组分系统。每个单独的双组分系统调节一组特定的基因的转录，这些基因对高渗胁迫有反应。
Microorganisms respond to hyperosmotic stress via changes in the levels of expression of large numbers of genes. Such responses are essential for acclimation to a new osmotic environment. To identify factors involved in the perception and transduction of signals caused by hyperosmotic stress, we examined the response of Synechocystis sp. PCC 6803, which has proven to be a particularly useful microorganism in similar analyses. We screened knockout libraries of histidine kinases (Hiks) and response regulators (Rres) in Synechocystis by DNA microarray and slot-blot hybridization analyses, and we identified several two-component systems, which we designated Hik-Rre systems, namely, Hik33-Rre31, Hik34-Rre1, and Hik10-Rre3, as well as Hik16-Hik41-Rre17, as the transducers of hyperosmotic stress. We also identified Hik2-Rre1 as a putative additional two-component system. Each individual two-component system regulated the transcription of a specific group of genes that were responsive to hyperosmotic stress.