First-trimester trophoblast cell model gene response to hypoxia
First-trimester trophoblast cell model gene response to hypoxia
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DOI：
10.1016/j.ajog.2006.01.067
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发表时间：
2006-03-01
影响因子：
9.8
通讯作者：
Lee, MJ
中科院分区：
文献类型：
--
作者：
Koklanaris, N;Nwachukwu, JC;Lee, MJ
目的：滋养层细胞的侵入，为胎盘形成和妊娠结局奠定了基础，可能发生在缺氧环境中。我们使用微阵列技术在滋养层细胞系，以确定缺氧反应基因，可能会影响placentation.Study设计：一个永生化的绒毛外细胞滋养层细胞系，HTR-8/SVneo，暴露于常氧（20%的氧气）或缺氧（1%的氧气）6小时。收集总RNA并制备用于微阵列研究。定量逆转录聚合酶链反应进行阵列confirmation.Results：我们证实了10缺氧反应基因的上调和下调，使用定量逆转录聚合酶链反应。被发现是缺氧反应的本体基因类别包括运动/迁移，血管生成，和apoptosis.Conclusion：特定的基因，被发现在这个头三个月的阵列（如纤溶酶原激活物抑制剂-1和金属蛋白酶组织抑制剂3）被上调已被描述在先兆子痫。我们发现的缺氧反应基因可能在妊娠早期是生理性的。然而，在怀孕后期这些相同基因的上调预示着不好。(c)2006 Mosby，Inc. All rights reserved.
Objective: Trophoblast invasion, which sets the stage for placentation and pregnancy outcome, likely occurs in a hypoxic environment. We used microarray technology in a trophoblast cell line to identify hypoxia-responsive genes that may impact placentation.Study design: An immortalized extravillous cytotrophoblast cell line, HTR-8/SVneo, was exposed to normoxia (20% oxygen) or hypoxia (1% oxygen) for 6 hours. Total RNA was harvested and prepared for microarray study. Quantitative reverse transcriptase polymerase chain reaction was performed for array confirmation.Results: We confirmed the up- and down-regulation of 10 hypoxia-responsive genes using quantitative reverse transcriptase polymerase chain reaction. Ontologic gene categories that were found to be hypoxia-responsive included motility/migration, angiogenesis, and apoptosis.Conclusion: Specific genes that were found to be up-regulated in this first-trimester array (Such as plasminogen activator inhibitor-1 and tissue inhibitor of metalloproteinase 3) have been described in preeclampsia. The hypoxia-responsive genes that we identified may be physiologic in early pregnancy. However, Up-regulation of these same genes in later pregnancy augurs poorly. (c) 2006 Mosby, Inc. All rights reserved.