Genetic diversity of the Junin virus in Argentina:: Geographic and temporal patterns
Genetic diversity of the Junin virus in Argentina:: Geographic and temporal patterns
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DOI：
10.1006/viro.2000.0345
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发表时间：
2000-06-20
期刊：
影响因子：
3.7
通讯作者：
St Jeort, SC
中科院分区：
文献类型：
--
作者：
García, JB;Morzunov, SP;St Jeort, SC
从阿根廷中部的39株细胞培养Junin病毒（JUN）中纯化RNA，其中包括人源和啮齿动物源分离株（分别为26株和13株），以及2株实验室Junin病毒XJ CI3和XJ #44。使用jun特异性引物扩增核衣壳蛋白基因的511个核苷酸（nt）片段和糖蛋白1 （GP1）基因的495个核苷酸片段。GP1基因在nt水平上的遗传多样性可达13%，氨基酸水平上的遗传多样性可达9%，NP基因的遗传多样性可达9% （nt）和4% （aa）。两个基因的系统发育分析显示了三个不同的分支。第一个进化支由来自疫区中心的JUN菌株组成，包括本研究分析的大部分JUN菌株。第二支包含4株JUN毒株，于1963年至1971年间从已知流行区的最西端科尔多瓦省分离出来。第三支包含4株JUN菌株，它们来自已知流行区东北边缘Zarate捕获的卡洛米斯肌鼠。从GenBank获得的某些JUN序列被鉴定为XJ、XJ #44和Candid #1菌株，似乎形成了一个单独的分支。对来自阿根廷出血热不同临床表现和结局患者的7株JUN菌株进行了超过400 nt的GP1和GP2基因测序。对相应的aa序列的分析不允许我们将任何特定的遗传标记归因于人类疾病的变化严重程度或临床情况。(C) 2000年学术出版社。
RNA was purified from 39 strains of cell-cultured Junin virus (JUN) from central Argentina, which included both human- and rodent-derived isolates (a total of 26 and 13, respectively), as well as from 2 laboratory JUN strains, XJ CI3 and XJ #44. JUN-specific primers were used to amplify a 511-nucleotide (nt) fragment of the nucleocapsid protein gene and a 495-nt fragment of the glycoprotein 1 (GP1) gene. Genetic diversity among JUN strains studied was up to 13% at the nt lever and up to 9% at the amino acid (aa) level for the GP1 gene and up to 9% (nt) and 4% (aa) for the NP gene. Phylogenetic analyses of both genes revealed three distinct clades. The first clade was composed of the JUN strains from the center of the endemic area and included the majority of JUN strains analyzed in the current study. The second clade contained 4 JUN strains isolated between 1963 and 1971 from Cordoba Province, the western-most edge of the known endemic area. The third clade contained 4 JUN strains that originated from Calomys musculinus trapped in Zarate, the northeastern edge of the known endemic area. Certain JUN sequences, which were obtained from GenBank and identified as XJ, XJ #44, and Candid #1 strains, appeared to form a separate clade. Over 400 nt of the GP1 and GP2 genes were additionally sequenced for 7 JUN strains derived from patients with different clinical presentations and outcomes of Argentine hemorrhagic fever. Analysis of the corresponding aa sequences did not allow us to attribute any particular genetic marker to the changing severity or clinical farm of the human disease. (C) 2000 Academic Press.