SecA homolog in protein transport within chloroplasts: evidence for endosymbiont-derived sorting.
SecA homolog in protein transport within chloroplasts: evidence for endosymbiont-derived sorting.
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DOI：
10.1126/science.7973633
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发表时间：
1994-11
期刊：
影响因子：
56.9
通讯作者：
Jianguo Yuan;Ralph Henry;M. Mccaffery;Kenneth Cline
中科院分区：
文献类型：
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作者：
Jianguo Yuan;Ralph Henry;M. Mccaffery;Kenneth Cline
SecA 蛋白是细菌蛋白易位机制中重要的叠氮化物敏感成分。现在在豌豆叶绿体中鉴定出的 SecA 蛋白同源物 (CPSecA) 已被纯化至均质。 CPSecA 支持蛋白质以叠氮化物敏感的方式转运到类囊体（叶绿体内膜网络）中。蛋白质转运至类囊体的三种途径中只有一种使用 CPSecA 机制。在叶绿体内蛋白质运输中使用细菌同源机制为叶绿体内蛋白质的保守分选提供了证据。
The SecA protein is an essential, azide-sensitive component of the bacterial protein translocation machinery. A SecA protein homolog (CPSecA) now identified in pea chloroplasts was purified to homogeneity. CPSecA supported protein transport into thylakoids, the chloroplast internal membrane network, in an azide-sensitive fashion. Only one of three pathways for protein transport into thylakoids uses the CPSecA mechanism. The use of a bacteria-homologous mechanism in intrachloroplast protein transport provides evidence for conservative sorting of proteins within chloroplasts.