Genotype ✕ Sucrose Interactions for Somatic Embryogenesis in Soybean
Genotype ✕ Sucrose Interactions for Somatic Embryogenesis in Soybean
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基因型 ✕ 蔗糖相互作用对大豆体细胞胚胎发生的影响
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
S. Oka
中科院分区：
文献类型：
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作者：
T. Komatsuda;K. Kaneko;S. Oka
体细胞胚胎发生是大豆[Glycine max（L.）Merr.]。本研究旨在测试大豆未成熟胚在几种蔗糖浓度下产生体细胞胚的能力。从16个大豆品种（G. max和G. gracilis Skvortz.）将品种培养在含有浓度为5、10、20和30 g L-1的蔗糖的胚胎发生培养基上。培养28 d后，计数每对子叶的体细胞胚数。方差分析表明，基因型、蔗糖浓度和基因型×蔗糖互作效应均达到极显著水平。通过每个品种对所有品种在每个蔗糖浓度下的平均值的回归，进一步分析了基因型×蔗糖互作
Somatic embryogenesis is a route of plant regeneration in soybean [Glycine max (L.) Merr.]. This study was undertaken to test the ability of immature soybean embryos to produce somatic embryos across several sucrose concentrations. Cotydelons isolated from immature embryos of 16 soybean (G. max and G. gracilis Skvortz.) cultivars were cultured on embryogenesis media containing sucrose at concentrations of 5, 10, 20, and 30g L −1 . After 28 d in culture, the number of somatic embryos per pair of cotydelons was counted. The analysis of variance showed that the effects of genotype, sucrose concentration, and genotype × sucrose interaction were all highly significant. The genotype × sucrose interaction was analyzed further by regression of each cultivar on the mean of all varieties for each sucrose concentration