Expressed nifH genes of endophytic bacteria detected in field-grown sweet potatoes (Ipomoea batatas L.)
Expressed nifH genes of endophytic bacteria detected in field-grown sweet potatoes (Ipomoea batatas L.)
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DOI：
10.1264/jsme2.23.89
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发表时间：
2008-01-01
影响因子：
2.2
通讯作者：
Fujihara, Shinsuke
中科院分区：
文献类型：
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作者：
Terakado-Tonooka, Junko;Ohwaki, Yoshinari;Fujihara, Shinsuke
我们研究了在田间生长的甘薯（Ipomoea batatas L.）中表达的内生固氮菌固氮酶还原酶（nifH）基因。通过逆转录（RT）-PCR。从种植后几个月田间种植的甘薯茎和贮藏根中获得的mRNA中扩增对应于nifH的基因片段。序列分析表明，这些克隆的nifH序列的慢生根瘤菌，Pelomonas，芽孢杆菌的DNA数据库中的同源性。从这些甘薯提取的基因组DNA扩增的nifH基因的调查也显示出高度的相似性，各种α-变形菌，包括慢生根瘤菌，β-变形菌，和蓝藻。这些结果表明，慢生根瘤菌定殖和表达nifH基因不仅在豆科植物的根瘤，但也在甘薯固氮内生菌。
We examined the nitrogenase reductase (nifH) genes of endophytic diazotrophic bacteria expressed in field-grown sweet potatoes (Ipomoea batatas L.) by reverse transcription (RT)-PCR. Gene fragments corresponding to nifH were amplified from mRNA obtained from the stems and storage roots of field-grown sweet potatoes several months after planting. Sequence analysis revealed that these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database. Investigation of the nifH genes amplified from the genomic DNA extracted from these sweet potatoes also showed high similarity to various alpha-proteobacteria including Bradyrhizobium, beta-proteobacteria, and cyanobacteria. These results suggest that bradyrhizobia colonize and express nifH genes not only in the root nodules of leguminous plants but also in sweet potatoes as diazotrophic endophytes.