Identification and functional analysis of three isoforms of bovine BST-2.
Identification and functional analysis of three isoforms of bovine BST-2.
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DOI：
10.1371/journal.pone.0041483
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yasuda J
中科院分区：
文献类型：
--
作者：
Takeda E;Nakagawa S;Nakaya Y;Tanaka A;Miyazawa T;Yasuda J
人BST-2（hBST-2）已被鉴定为细胞抗病毒因子，其阻断各种包膜病毒的释放。BST-2的直向同源物已经在包括人、猴、猪、小鼠、猫和羊的几个物种中鉴定。据报道，所有这些都具有抗病毒活性。在绵羊中观察到BST-2基因的重复，旁系同源物被称为绵羊BST-2A和BST 2-B，尽管在大多数物种中仅鉴定出对应于BST-2的单个基因。在这项研究中，我们确定了三个亚型的牛BST-2，命名为bBST-2A 1，bBST-2A 2和bBST-2B，在牛细胞与I型干扰素处理，但没有在未经处理的细胞。bBST-2A 1和bBST-2A 2都通过N-连接的糖基化和GPI-锚以及hBST-2进行后修饰，而bBST-2B没有这些修饰。bBST-2A 1和bBST-2A 2的外源表达显著降低了细胞中牛白血病病毒和水泡性口炎病毒的产生，而bBST-2B的抗病毒活性远低于bBST-2A 1和bBST-2A 2。我们的数据表明，bBST-2A 1和bBST-2A 2功能的一部分，干扰素诱导的先天免疫对病毒感染。另一方面，bBST-2B可能具有与bBST-2A 1和bBST-2A 2不同的生理功能。
Human BST-2 (hBST-2) has been identified as a cellular antiviral factor that blocks the release of various enveloped viruses. Orthologues of BST-2 have been identified in several species, including human, monkeys, pig, mouse, cat and sheep. All have been reported to possess antiviral activity. Duplication of the BST-2 gene has been observed in sheep and the paralogues are referred to as ovine BST-2A and BST2-B, although only a single gene corresponding to BST-2 has been identified in most species. In this study, we identified three isoforms of bovine BST-2, named bBST-2A1, bBST-2A2 and bBST-2B, in bovine cells treated with type I interferon, but not in untreated cells. Both bBST-2A1 and bBST-2A2 are posttranslationally modified by N-linked glycosylation and a GPI-anchor as well as hBST-2, while bBST-2B has neither of these modifications. Exogenous expression of bBST-2A1 or bBST-2A2 markedly reduced the production of bovine leukemia virus and vesicular stomatitis virus from cells, while the antiviral activity of bBST-2B was much weaker than those of bBST-2A1 and bBST-2A2. Our data suggest that bBST-2A1 and bBST-2A2 function as part of IFN-induced innate immunity against virus infection. On the other hand, bBST-2B may have a different physiological function from bBST-2A1 and bBST-2A2.
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影响因子：
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作者：
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作者：
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通讯作者：
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