Direct effect of basic fibroblast growth factor on gene transcription in a cell-free system.
Direct effect of basic fibroblast growth factor on gene transcription in a cell-free system.
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碱性成纤维细胞生长因子对无细胞系统中基因转录的直接影响。
DOI：
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发表时间：
1992
影响因子：
11.1
通讯作者：
Katsuzo NISHIKAWAt
中科院分区：
文献类型：
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作者：
Y. Nakanishi;K. Kihara;K. Mizuno;Y. Masamune;Y. Yoshitake;Katsuzo NISHIKAWAt
最近关于完整的成纤维细胞生长因子(成纤维细胞生长因子)移位到细胞核的研究结果表明，它在核事件中直接发挥作用。我们研究了人碱性成纤维细胞生长因子(BFGF)在无细胞系统中对基因转录的影响。用艾氏腹水癌细胞的核提取液转录小鼠编码磷酸甘油酸激酶1和2(PGK-1和PGK-2)的基因时，成纤维细胞生长因子对转录有不同的影响：在碱性成纤维细胞生长因子的存在下，PGK-1基因的转录被抑制，而PGK-2基因的转录被增强。在检测病毒基因时，bFGF对腺病毒主要晚期DNA的转录有轻微的刺激作用，对腺病毒早期E1a DNA和人类免疫缺陷病毒DNA的转录没有影响。此外，pGK-2基因的5‘上游区域含有负顺式作用元件，这是碱性成纤维细胞生长因子进行转录刺激所必需的。这些结果表明，碱性成纤维细胞生长因子可以通过基因特异性的方式直接在细胞核内调节转录。
Recent findings on the translocation of intact fibroblast growth factor (FGF) into the cell nucleus suggest that it functions directly in nuclear events. We examined the effect of human basic FGF (bFGF) on gene transcription in a cell-free system. When mouse genes encoding phosphoglycerate kinases 1 and 2 (Pgk-1 and Pgk-2) were transcribed by using nuclear extracts of Ehrlich ascites tumor cells, FGF affected transcription in different ways: in the presence of bFGF, transcription of the Pgk-1 gene was inhibited, whereas that of the Pgk-2 gene was enhanced. When viral genes were tested, transcription of the adenovirus major late DNA was slightly stimulated but that of the adenovirus early E1A DNA or the human immunodeficiency virus DNA was not changed by the addition of bFGF. Moreover, the presence of a distinct 5' upstream region of the Pgk-2 gene, which includes a negative cis-acting element, was required for transcription stimulation by bFGF. These results suggest that bFGF can regulate transcription directly in the nucleus in a gene-specific manner.