Highly selective fluorometric probes for detection of HClO in livingcells
Highly selective fluorometric probes for detection of HClO in livingcells
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用于检测活细胞中 HClO 的高选择性荧光探针
DOI：
10.1016/j.snb.2018.03.124
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jianguo Fang
中科院分区：
文献类型：
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作者：
Jinyu Sun;Liangwei Zhang;Yuesong Hu;Jianguo Fang
由于苯并呋咱（NBD）荧光团具有突出的光化学和物理性质，是构建荧光传感器的常用平台。本文报道了一种通过调节苯环上的推拉对来调节NBD荧光的策略，得到了两种新型的荧光探针MeS-DMAB和EtS-DMAB，它们可以选择性地检测水溶液中的次氯酸（HOCl），具有较大的Stokes位移（~ 170 nm）。介绍了这两种探针的合成和光学性质。对HOCl与MeS-DMAB的反应产物进行了分离和表征。结果表明，硫醚氧化成相应的亚砜是开启荧光的原因。最后，成功地将该探针用于活细胞中外源性和内源性HOCl的成像。
Due to the prominent photochemical and photophysical properties, the benzofurazan (NBD) fluorophore is a popular platform for constructing fluorescencet sensors. We reported herein a strategy to tune the fluorescence of NBD by modulating the push-pull pairs on the benzene ring, and obtained two novel fluorescent probes,MeS-DMABandEtS-DMAB, which can selectively detect hypochlorous acid (HOCl) over other reactive oxygen species in aqueous solution with a large stokes shift (∼170 nm). The synthesis and optical properties of these two probes were described. The reaction products of HOCl withMeS-DMABwere isolated and characterized. It turns out that oxidation of the thioether to the corresponding sulfoxide accounts for the turn-on fluorescence. Finally, the probeEtS-DMABwas successfully applied to image exogenous and endogenous HOCl in live cells.