MOLECULAR ANALYSIS OF THE ASSOCIATION OF HLA-B53 AND RESISTANCE TO SEVERE MALARIA
MOLECULAR ANALYSIS OF THE ASSOCIATION OF HLA-B53 AND RESISTANCE TO SEVERE MALARIA
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DOI：
10.1038/360434a0
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发表时间：
1992-12-03
期刊：
影响因子：
64.8
通讯作者：
WHITTLE, HC
中科院分区：
文献类型：
--
作者：
HILL, AVS;ELVIN, J;WHITTLE, HC
通过对人类白细胞抗原HLAB53洗脱的多肽进行测序，并在生化和细胞学分析中筛选恶性疟原虫红细胞前期抗原的候选表位，研究了人类白细胞抗原HLAB53与严重疟疾之间的保护作用。在疟疾免疫的非洲人中，人类白细胞抗原-B53限制的细胞毒性T淋巴细胞识别肝期特异性抗原-1(LSA-1)中的保守的非特异性多肽，但在其他抗原中没有发现人类白细胞抗原-B53限制的表位。这些发现表明了这种人类白细胞抗原-疾病关联的可能的分子基础，并支持肝期特异性抗原-1作为疟疾疫苗成分的候选。
The protective association between the human leukocyte antigen HLA-B53 and severe malaria was investigated by sequencing of peptides eluted from this molecule followed by screening of candidate epitopes from pre-erythrocytic-stage antigens of Plasmodium falciparum in biochemical and cellular assays. Among malaria-immune Africans, HLA-B53-restricted cytotoxic T lymphocytes recognized a conserved nonamer peptide from liver-stage-specific antigen-1 (LSA-1), but no HLA-B53-restricted epitopes were identified in other antigens. These findings indicate a possible molecular basis for this HLA-disease association and support the candidacy of liver-stage-specific antigen-1 as a malaria vaccine component.