EARLY PHENOTYPIC ACTIVATION OF CIRCULATING HELPER MEMORY T-CELLS IN SCLERODERMA - CORRELATION WITH DISEASE-ACTIVITY
EARLY PHENOTYPIC ACTIVATION OF CIRCULATING HELPER MEMORY T-CELLS IN SCLERODERMA - CORRELATION WITH DISEASE-ACTIVITY
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DOI：
10.1136/ard.52.4.272
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发表时间：
1993-04-01
影响因子：
27.4
通讯作者：
TODESCO, S
中科院分区：
文献类型：
--
作者：
FIOCCO, U;ROSADA, M;TODESCO, S
目的-几种辅助/激活分子的差异表达（CD26，CD29，CD45RA，CD25，MLR4，比较硬皮病患者外周血CD 4+和CD 8 + T淋巴细胞表面HLA-DR抗原的表达情况，并与正常对照组和其他结缔组织系统性疾病患者进行比较，以寻找T细胞参与硬皮病发病过程的证据。本文用一组单克隆抗体和流式细胞术分析了17例硬皮病、10例系统性红斑狼疮和5例类风湿关节炎患者外周血T细胞表面分子的双色表达，并与10例正常人对照。在这些硬皮病患者中，进一步比较了活动期和静止期患者的双色T CD 4+表型。8例硬皮病患者的CD 4 + T细胞表面分子的共表达也通过三色流式细胞术进行了分析。结果硬皮病患者的CD 4 + CD 26+和CD 4 + CD 25+的百分比和绝对数增加，而CD 3 + CD 29+的百分比减少。此外，观察到较高的CD 4 + CD 26 + T细胞百分比和绝对细胞数与疾病活动性之间存在显著相关性。大多数硬皮病患者的CD 4+外周血T细胞显示的CD 26 + CD 45 RA-表型通过三色流式细胞术analysis. Conclusions早期辅助记忆T细胞活化的独特模式，在这些患者迅速发展硬皮病支持的作用，T细胞介导的机制在硬皮病的进展。
Objectives-The differential expression of several accessory/activation molecules (CD26, CD29, CD45RA, CD25, MLR4, HLA-DR) on peripheral blood CD4+ and CD8+ T lymphocytes in patients with scleroderma was compared with that in controls and patients with other connective systemic diseases to look for evidence of the involvement of T cells in the disease process of scleroderma.Methods-The two colour expression of surface molecules by circulating T cells was analysed with a panel of monoclonal antibodies and flow cytometry in 17 patients with scleroderma, 10 patients with systemic lupus erythematosus, and five patients with rheumatoid arthritis, and the results compared with those for 10 normal controls. The two colour T CD4+ phenotype was further compared between patients with active and quiescent disease in these patients with scleroderma. The coexpression of surface molecules by CD4+ T cells was also analysed by three colour flow cytometry in eight patients with scleroderma.Results-Patients with scleroderma showed increased CD4+CD26+ and CD4+CD25+ percentages and absolute numbers and decreased CD3+CD29+ percentages compared with controls. Moreover, a significant correlation between the higher CD4+CD26+ T cell percentage and absolute cell numbers with disease activity was observed. Most of the CD4+ peripheral blood T cells from patients with scleroderma showed the CD26+CD45RA-phenotype by three colour flow cytometry analysis.Conclusions-The distinctive pattern of early helper memory T cell activation in these patients with rapidly evolving scleroderma supports the role of a T cell mediated mechanism in the progression of scleroderma.