Longitudinal study of immune response to Pseudomonas aeruginosa antigens in cystic fibrosis
Longitudinal study of immune response to Pseudomonas aeruginosa antigens in cystic fibrosis
复制标题
囊性纤维化中铜绿假单胞菌抗原免疫反应的纵向研究
作者：
G. Döring;N. Høiby
在10年的时间里，10例囊性纤维化患者的临床状况进行了评估的基础上，每月测量肺功能和体重，血清碱性蛋白酶和弹性蛋白酶抗体滴度和铜绿假单胞菌标准抗原的沉淀素的数量测定用放射免疫法和交叉免疫电泳。在体外测量来自患者的铜绿假单胞菌菌株的碱性蛋白酶和弹性蛋白酶浓度。几乎所有患者在慢性铜绿假单胞菌肺部感染发作后多年的免疫应答增加，表明铜绿假单胞菌肺部感染期间这些抗原的产生未受损，而临床状态下降。碱性蛋白酶的平均免疫反应时间为15个月，弹性蛋白酶为11个月，标准抗原为6个月。
During a 10-year period, the clinical states of 10 cystic fibrosis patients were evaluated on the basis of monthly measurement of lung function and weight; serum antibody titers to alkaline protease and elastase and the number of precipitins to Pseudomonas aeruginosa standard antigen were determined by radioimmunoassay and crossed immunoelectrophoresis. Alkaline protease and elastase concentrations of the P. aeruginosa strains from the patients were measured in vitro. The immune response increased in nearly all patients after the onset of chronic P. aeruginosa lung infection over years, suggesting unimpaired production of these antigens during P. aeruginosa lung infection, whereas the clinical states declined. The mean time for immune response was 15 months for alkaline protease, 11 months for elastase, and 6 months for standard antigen.