Differential expression and functions of Ehm2 transcript variants in lung adenocarcinoma
Differential expression and functions of Ehm2 transcript variants in lung adenocarcinoma
复制标题
Ehm2转录变异体在肺腺癌中的差异表达和功能
DOI：
10.3892/ijo.2019.4732
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发表时间：
2019-05-01
影响因子：
5.2
通讯作者：
Jiang, Wen G.
中科院分区：
文献类型：
--
作者：
Li, Shenglan;Ma, Jian;Jiang, Wen G.
Ehm 2（也称为红细胞膜蛋白带4.1样蛋白4 B（EPB 41 L4 B））是NF 2/ERM/4.1超家族的成员。在转移性癌细胞中已经观察到Ehm 2的过表达。通过选择性剪接，Ehm 2基因产生两种转录变体，编码两种不同的同种型，Ehm 2/1和Ehm 2/2。这些不同的Ehm 2转录变体的生物学功能仍不清楚。本研究旨在确定Ehm 2变异体在肺腺癌中的表达及其在患者疾病进展中的作用。采用免疫组化和western blot分析Ehm 2转录变体在人肺腺癌组织中的表达。Ehm 2变体在A549人肺腺癌细胞中过表达或被敲低。然后使用体外细胞活力、侵袭和迁移测定来检查遗传修饰对肺癌细胞的细胞功能的后续影响。免疫印迹法检测上皮-间质转化（EMT）相关标志物的表达。使用Kaplan-Meier生存分析来分析Ehm 2变体表达与患者生存的关联。Ehm 2/1在肺癌组织中的表达明显低于配对的正常肺组织（P
Ehm2 [also known as erythrocyte membrane protein band 4.1-like protein 4B (EPB41L4B)] is a member of the NF2/ERM/4.1 superfamily. The overexpression of Ehm2 has been observed in metastatic cancer cells. Through alternative splicing, the Ehm2 gene produces two transcript variants that encode the two different isoforms, Ehm2/1 and Ehm2/2. The biological functions of these different Ehm2 transcript variants remain unclear. The present study aimed to determine the expression of the Ehm2 variants in lung adenocarcinoma and their involvement in the disease progression of the patients. The expression of Ehm2 transcript variants in human lung adenocarcinoma tissues was analyzed using immunohistochemistry and western blot analysis. Ehm2 variants were overexpressed or knocked down in A549 human lung adenocarcinoma cells. The consequent effects of the genetic modifications on the cellular functions of lung cancer cells were then examined using in vitro cell viability, invasion and migration assays. The expression of epithelial-mesenchymal transition (EMT)-related markers was evaluated by western blot analysis in the cell models. The association of Ehm2 variant expression with patient survival was analyzed using Kaplan-Meier survival analysis. The expression of Ehm2/1 was significantly decreased in lung cancers compared with the paired normal lung tissues (P