HAX-1 overexpression, splicing and cellular localization in tumors.
HAX-1 overexpression, splicing and cellular localization in tumors.
复制标题
肿瘤中 HAX-1 过度表达、剪接和细胞定位。
DOI：
10.1186/1471-2407-10-76
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发表时间：
2010-03-02
期刊：
影响因子：
3.8
通讯作者：
Grzybowska EA
中科院分区：
文献类型：
--
作者：
Trebinska A;Rembiszewska A;Ciosek K;Ptaszynski K;Rowinski S;Kupryjanczyk J;Siedlecki JA;Grzybowska EA
HAX-1已被描述为潜在参与癌发生，特别是转移的蛋白质。其参与细胞凋亡和细胞迁移的调节，沿着一些数据表明其在癌细胞系和肿瘤中的过表达，表明HAX-1可能在肿瘤转化中起作用。在这里，我们提出了第一个系统的分析HAX-1在几个实体瘤的表达。使用定量RT-PCR，我们已经确定了HAX 1剪接变体I在几个实体瘤的mRNA水平。我们还通过半定量和定量RT-PCR分析了5个HAX-1剪接变异体在乳腺癌样本和来自相同个体的正常组织中的表达。定量PCR检测雌激素对乳腺癌细胞株HAX 1表达的影响。对正常和乳腺癌样品进行HAX-1的免疫组织化学分析。结果揭示了HAX 1在乳腺癌、肺癌和黑色素瘤中具有统计学意义的上调，沿着剪接模式的一些微小变化。乳腺癌样品中的HAX-1上调通过免疫组织化学分析证实，其还揭示了肿瘤细胞核中有趣的HAX-1定位，与强ER状态相关。HAX-1在癌组织中的水平升高表明其参与肿瘤转化，特别是在乳腺癌中。乳腺癌样本中HAX-1核定位和ER状态之间的联系仍有待澄清。
HAX-1 has been described as a protein potentially involved in carcinogenesis and especially metastasis. Its involvement in regulation of apoptosis and cell migration along with some data indicating its overexpression in cancer cell lines and tumors suggests that HAX-1 may play a role in neoplastic transformation. Here we present the first systematic analysis of HAX-1 expression in several solid tumors. Using quantitative RT-PCR, we have determined the mRNA levels of HAX1 splice variant I in several solid tumors. We have also analyzed by semiquantitative and quantitative RT-PCR the expression of five HAX-1 splice variants in breast cancer samples and in normal tissue from the same individuals. Quantitative PCR was also employed to analyze the effect of estrogen on HAX1 expression in breast cancer cell line. Immunohistochemical analysis of HAX-1 was performed on normal and breast cancer samples. The results reveal statistically important HAX1 up-regulation in breast cancer, lung cancer and melanoma, along with some minor variations in the splicing pattern. HAX-1 up-regulation in breast cancer samples was confirmed by immunohistochemical analysis, which also revealed an intriguing HAX-1 localization in the nuclei of the tumor cells, associated with strong ER status. HAX-1 elevated levels in cancer tissues point to its involvement in neoplastic transformation, especially in breast cancer. The connection between HAX-1 nuclear location and ER status in breast cancer samples remains to be clarified.
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影响因子：
4.8
作者：
Kasashima, Katsumi;Ohta, Eriko;Endo, Hitoshi
通讯作者：
Endo, Hitoshi
影响因子：
4.8
作者：
Rhodes, DR;Yu, JJ;Chinnaiyan, AM
通讯作者：
Chinnaiyan, AM
影响因子：
11.1
作者：
Carlsson, G.;van't Hooft, I.;Henter, J-I
通讯作者：
Henter, J-I
DOI：
10.1073/pnas.97.8.4017
发表时间：
2000-04-11
影响因子：
11.1
作者：
Gallagher, AR;Cedzich, A;Witzgall, R
通讯作者：
Witzgall, R
影响因子：
3.9
作者：
BROSSART, P;KEILHOLZ, U;HUNSTEIN, W
通讯作者：
HUNSTEIN, W