Colonial morphology of Burkholderia cepacia complex genomovar III:: Implications in exopolysaccharide production, pilus expression, and persistence in the mouse
Colonial morphology of Burkholderia cepacia complex genomovar III:: Implications in exopolysaccharide production, pilus expression, and persistence in the mouse
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DOI：
10.1128/iai.71.2.904-909.2003
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发表时间：
2003-02-01
影响因子：
3.1
通讯作者：
Speert, DP
中科院分区：
文献类型：
--
作者：
Chung, JW;Altman, E;Speert, DP
本研究的目的是确定洋葱伯克霍尔德氏菌复合体（BCC）的菌落形态在小鼠肺部感染模型中致病性的作用。BCC菌株C1394在鼻内激发后被白细胞减少小鼠迅速清除，而通过基因分型与亲本菌株难以区分的自发变体（C1394mp2）在肺中持续存在，并且在菌落形态上不同。亲本菌株具有哑光的菌落表型，产生很少的胞外多糖（EPS），并且具有轻微的纤毛。该变异体有光泽的表型，产生丰富的EPS，并且有大量的纤毛。通过在42 ℃下生长诱导无光泽到有光泽的菌落转化。BCC菌株变异体中的菌落形态与肺激发后的持久性相关，并且似乎与推定的毒力决定因素的阐述相关。
The purpose of this study was to determine the role of colonial morphology of Burkholderia cepacia complex (BCC) organisms in pathogenicity in a mouse model of pulmonary infection. BCC strain C1394 was rapidly cleared by leukopenic mice after intranasal challenge, whereas a spontaneous variant (C1394mp2) that was indistinguishable from the parent strain by genetic typing persisted in the lungs and differed in colonial morphology. The parent strain had a matte colonial phenotype, made scant exopolysaccharide (EPS), and was lightly piliated. The variant had a shiny phenotype, produced abundant EPS, and was heavily piliated. Matte to shiny colonial transformation was induced by growth at 42degreesC. Colonial morphology in the BCC strain variant was associated with persistence after pulmonary challenge and appeared to be correlated with the elaboration of putative virulence determinants.