Isolation of a new feline sarcoma virus (HZ1-FeSV): biochemical and immunological characterization of its translation product.
Isolation of a new feline sarcoma virus (HZ1-FeSV): biochemical and immunological characterization of its translation product.
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一种新的猫肉瘤病毒（HZ1-FeSV）的分离：其翻译产物的生化和免疫学特征。
DOI：
10.1016/0042-6822(84)90104-1
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
HardyJr,WD
中科院分区：
文献类型：
--
作者：
SnyderJr,HW;Singhal,MC;Zuckerman,EE;HardyJr,WD
从一只患有多中心纤维肉瘤的4岁家猫中分离到一株新的猫肉瘤病毒，命名为HZ 1-FeSV。建立了原代肿瘤细胞系，发现该细胞系产生的病毒在组织培养物中的猫胚胎肺成纤维细胞（FLF 3）和水貂肺成纤维细胞（CCL 64）中诱导病灶，并在接种的10周龄小猫中诱导纤维肉瘤。含有辅助病毒可挽救的病灶形成活性的FLF 3和CCL 64细胞的转化非生产性克隆的衍生表明HZ 1-FeSV复制缺陷。HZ 1-FeSV基因组在培养细胞中唯一可辨别的翻译产物是100，000-Da多聚蛋白（P100），其含有与肉瘤病毒特异性结构域共价连接的FeLVgag基因前体蛋白的氨基末端序列。含有P100的免疫沉淀物显示出能够磷酸化P100的酪氨酸残基的蛋白激酶活性。免疫学上，P100是高度交叉反应与gag-fespolyproteins编码的两个先前的特征性菌株的FeSV，GA-和ST-FeSV。通过比较含甲硫氨酸的胰蛋白酶肽，显示HZ 1-FeSV蛋白与GA-FeSV蛋白的关系比与ST-FeSV蛋白的关系更密切，但与其他两种蛋白质有区别。
A new strain of feline sarcoma virus, designated HZ1-FeSV, was isolated from a 4-year-old domestic cat with multicentric fibrosarcoma. A primary tumor cell line was established and virus produced from that line was found to induce foci in feline embryonic lung fibroblasts (FLF3) and mink lung fibroblasts (CCL64) in tissue culture and fibro-sarcomas in inoculated 10-week-old kittens. The derivation of transformed nonproducer clones of FLF3 and CCL64 cells containing helper virus-rescuable, focus-forming activity indicated that HZ1-FeSV was defective for replication. The only discernible translation product of the HZ1-FeSV genome in cultured cells was a 100,000-Da polyprotein (P100) which contained amino-terminal sequences of the FeLVgaggene precursor protein covalently linked to a sarcoma virus-specific domain. Immunoprecipitates containing P100 exhibited a protein kinase actvity capable of phosphorylating tyrosine residues of P100. Immunologically, P100 was highly cross-reactive withgag-fespolyproteins encoded by two previously characterized strains of FeSV, the GA- and the ST-FeSV. By comparison of methionine-containing tryptic peptides, the HZ1-FeSV protein was shown to be more closely related to the GA-FeSV protein than to the ST-FeSV protein, but to be distinguishable from both other proteins.