Importance of Akt signaling pathway for apoptosis in SARS-CoV-infected Vero E6 cells.
Importance of Akt signaling pathway for apoptosis in SARS-CoV-infected Vero E6 cells.
复制标题
DOI：
10.1016/j.virol.2004.07.005
复制
发表时间：
2004-10-01
期刊：
影响因子：
3.7
通讯作者：
Morikawa S
中科院分区：
文献类型：
--
作者：
Mizutani T;Fukushi S;Saijo M;Kurane I;Morikawa S
严重急性呼吸道综合征（SARS）是一种与新型冠状病毒（SARS-CoV）相关的急性呼吸道传染病。我们最近的研究表明，SARS冠状病毒感染诱导p38丝裂原活化蛋白激酶（MAPK）信号通路的激活和p38 MAPK抑制剂部分抑制其在Vero E6细胞的细胞病变效应。本研究的结果表明，在细胞死亡之前，Akt，这是一种凋亡抑制剂，也被激活，以响应病毒复制。Akt上的丝氨酸残基的磷酸化至少在感染后8小时（hpi）检测到，18 hpi后下降。因此，磷脂酰肌醇3-激酶（PI 3 K）/Akt途径在病毒感染的Vero E6细胞中被激活。然而，苏氨酸残基未被磷酸化。Akt的下游靶点糖原合成酶激酶3β（GSK-3β）被轻微磷酸化，表明Akt的活化水平非常低。在病毒感染的细胞中，PI 3 K通路下游的PKC β也被磷酸化。这些结果表明，Akt的弱激活不能阻止SARS-CoV感染诱导的Vero E6细胞凋亡。
Severe acute respiratory syndrome (SARS) is an acute respiratory tract infectious disease that is associated with a new coronavirus (SARS-CoV). Our recent study indicated that SARS-CoV infection induces activation of the p38 mitogen-activated protein kinase (MAPK) signaling pathway and the p38 MAPK inhibitor partially inhibited its cytopathic effect in Vero E6 cells. The results of the present study indicated that before cell death, Akt, which is an inhibitor of apoptosis, was also activated in response to viral replication. Phosphorylation of a serine residue on Akt was detected at least 8 h postinfection (hpi), which declined after 18 hpi. Thus, the phosphatidylinositol 3-kinase (PI3K)/Akt pathway is activated in virus-infected Vero E6 cells. However, a threonine residue was not phosphorylated. A downstream target of Akt, glycogen synthase kinase 3β (GSK-3β), was slightly phosphorylated, indicating that the level of activation of Akt was very low. PKCζ, which is downstream of the PI3K pathway, was also phosphorylated in virus-infected cells. These results suggested that weak activation of Akt cannot prevent apoptosis induced by SARS-CoV infection in Vero E6 cells.
登录
查看更多内容
DOI：
10.1016/j.bbrc.2004.05.107
发表时间：
2004-07-09
影响因子：
3.1
作者：
Mizutani T;Fukushi S;Saijo M;Kurane I;Morikawa S
通讯作者：
Morikawa S
影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
影响因子：
4
作者：
Juretic, N;Santibáñez, JF;Martínez, J
通讯作者：
Martínez, J
影响因子：
8.8
作者：
Liu, B;Fang, M;Fan, Z
通讯作者：
Fan, Z
影响因子：
2.6
作者：
Mizutani, T;Kobayashi, M;Takashima, I
通讯作者：
Takashima, I