Activation of N-methyl-D-aspartate receptor induces a shift of drebrin distribution:: Disappearance from dendritic spines and appearance in dendritic shafts
Activation of N-methyl-D-aspartate receptor induces a shift of drebrin distribution:: Disappearance from dendritic spines and appearance in dendritic shafts
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DOI：
10.1016/j.mcn.2005.11.003
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Shirao, T
中科院分区：
文献类型：
--
作者：
Sekino, Y;Tanaka, S;Shirao, T
树突棘是一种主要的肌动蛋白丝结合蛋白，定位于成熟的树突棘。最近的一项体内免疫电镜研究表明，在每个树突棘的drexylase含量的调节一些未知的机制。在目前的体外研究中，我们研究了谷氨酸刺激是否会改变树突棘中的drexate含量。谷氨酸刺激诱导树突棘的dreplasmid免疫染色消失，但导致树突轴和胞体的dreplasmid免疫染色的外观。NMDA受体拮抗剂可阻断谷氨酸诱导的drepletin免疫染色移位。免疫印迹分析表明，无论是总的和胞质dreplant保持不变，并揭示dreplant的转变是不是由于dreplant降解。这些结果表明，NMDA受体激活诱导的移位与肌动蛋白丝的drepletion的亚细胞分布，这种移位可能是伴随着突触可塑性的肌动蛋白重组的分子基础。(c)2005年爱思唯尔公司All rights reserved.
Drebrin is a major actin-filament-binding protein localized in mature dendritic spines. A recent in vivo immunoelectron microscopic study suggests that drebrin content at each dendritic spine is regulated by some unknown mechanisms. In the present in vitro study, we examined whether glutamate stimulation alters drebrin content in dendritic spines. Glutamate stimulation induced disappearance of drebrin immunostaining from dendritic spines but led to appearance of drebrin immunostaining in dendritic shafts and somata. The glutamate-induced shift of drebrin immunostaining was blocked by an NMDA receptor antagonist. Immunoblot analyses showed that both the total and the cytosolic drebrin remained unchanged and revealed that the drebrin shift was not due to drebrin degradation. These findings indicate that NMDA receptor activation induces a shift in subcellular distribution of drebrin associated with actin filaments, and that the shift might be a molecular basis for actin reorganization accompanied with synaptic plasticity. (c) 2005 Elsevier Inc. All rights reserved.