Release of lipopolysaccharide from intracellular compartments containing Salmonella typhimurium to vesicles of the host epithelial cell
Release of lipopolysaccharide from intracellular compartments containing Salmonella typhimurium to vesicles of the host epithelial cell
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DOI：
10.1128/iai.65.1.24-34.1997
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发表时间：
1997-01-01
影响因子：
3.1
通讯作者：
Finlay, BB
中科院分区：
文献类型：
--
作者：
GarciadelPortillo, F;Stein, MA;Finlay, BB
细菌脂多糖（LPS）对真核细胞的生物学效应传统上是通过LPS对敏感细胞的胞外攻击来表征的。在这项研究中，我们报告的能力，鼠伤寒沙门氏菌释放LPS一旦它位于培养的上皮细胞的细胞内环境。LPS从胞内细菌所在的液泡区室释放到宿主细胞胞质溶胶中存在的囊泡。当入侵细菌进入宿主细胞时，在感染细胞中检测到囊泡相关的LPS。LPS的释放仅限于S.在鼠伤寒沙门氏菌感染的细胞中没有观察到LPS，这表明LPS的传播完全发生在感染细胞的细胞内环境中。当感染细胞的细胞内环境中存在LPS时，宿主囊泡中存在的LPS的量达到最大值。当胞内S.鼠伤寒沙门氏菌细胞开始增殖，此时整个宿主细胞胞质溶胶充满了大量含有LPS的囊泡。所有这些数据支持细胞内细菌病原体可能通过释放生物活性细菌组分如LPS从细胞内位置向宿主细胞发出信号的概念。
The biological effects of bacterial lipopolysaccharide (LPS) on eucaryotic cells have traditionally been characterized following extracellular challenge of LPS on susceptible cells. In this study, we report the capacity of Salmonella typhimurium to release LPS once it is located in the intracellular environment of cultured epithelial cells. LPS is liberated from vacuolar compartments, where intracellular bacteria reside, to vesicles present in the host cell cytosol. The vesicle-associated LPS is detected in infected cells from the time when invading bacteria enter the host cell. Release of LPS is restricted to S. typhimurium-infected cells, with no LPS observed in neighboring uninfected cells, suggesting that dissemination of LPS occurs entirely within the intracellular environment of the infected cell. The amount of LPS present in host vesicles reaches a maximum when intracellular environment of the infected cell. The amount of LPS present in host vesicles reaches a maximum when intracellular S. typhimurium cells start to proliferate, a time at which the entire host cell cytosol is filled with numerous vesicles containing LPS. All these data support the concept the intracellular bacterial pathogens might signal the host cell from intracellular locations by releasing bioactive bacterial components such as LPS.