Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme.
Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme.
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DOI：
10.1152/ajpendo.1985.249.1.e107
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发表时间：
1985-07
期刊：
影响因子：
--
通讯作者：
P. Iverius;J. Brunzell
中科院分区：
文献类型：
--
作者：
P. Iverius;J. Brunzell
利用三种不同的方法从人脂肪组织活检标本中恢复脂蛋白脂肪酶（LPL）活性的测定程序已经开发出来。用含肝素和血清的生理缓冲液在4℃和37℃下从小块组织中洗脱酶。在洗涤剂（脱氧胆酸钠和Nonidet P-40）存在的情况下，从组织匀浆中提取酶，这明显提高了酶活性的恢复。这表明，4℃洗脱仅代表细胞外酶活性，因此理论上是血管内皮生理活性LPL的最接近测量，而37℃洗脱还反映了孵育期间分泌的一些细胞内酶。在不同相对体重的女性受试者中，37℃洗脱的活性以及洗涤剂萃取物活性与4℃洗脱的细胞外活性高度相关（r = 0.9）。此外，这三个参数与肝素后血浆中LPL活性密切相关，表明它们是LPL生理活性的有效指标。肝素输注脂肪组织60分钟后血浆中LPL活性的回归结果显示，4℃和37℃洗脱后LPL活性的相关系数（r = 0.725和0.754）高于洗涤剂提取后的相关系数（r = 0.607）。此外，饲喂后脂肪组织LPL的增量（34%）是在4和37℃下洗脱的活性（19%）的两倍左右。（摘要删节250字）
An assay procedure using three different methods to recover lipoprotein lipase (LPL) activity from biopsy specimens of human adipose tissue has been developed. Elution of enzyme from small pieces of tissue was performed at 4 and 37 degrees C using a physiological buffer containing heparin and serum. Extraction of enzyme from a tissue homogenate was carried out in the presence of detergent (sodium deoxycholate and Nonidet P-40), which markedly improved the recovery of enzyme activity. It is suggested that elution at 4 degrees C represents extracellular enzyme activity only and therefore theoretically is the closest measure of physiologically active LPL on vascular endothelium, whereas elution at 37 degrees C, in addition, reflects some intracellular enzyme secreted during the incubation period. In female subjects of various relative body weights activity eluted at 37 degrees C as well as detergent-extracted activity were highly correlated with the extracellular activity eluted at 4 degrees C (r = 0.9). Furthermore, all three parameters correlated strongly with LPL activity in post-heparin plasma, suggesting that they are valid indices of physiologically active LPL. The regression of LPL activity in plasma after a 60-min heparin infusion on adipose tissue LPL yielded higher correlation coefficients for activities recorded after elution at 4 and 37 degrees C (r = 0.725 and 0.754, respectively) than for detergent extraction (r = 0.607). Moreover, the increment of adipose tissue LPL after feeding was approximately twice as high for the activity eluted at 4 and 37 degrees C (34%) as for detergent-extracted activity (19%).(ABSTRACT TRUNCATED AT 250 WORDS)