An analysis of monocytes and dendritic cells differentiated from human peripheral blood monocyte-derived induced pluripotent stem cells
An analysis of monocytes and dendritic cells differentiated from human peripheral blood monocyte-derived induced pluripotent stem cells
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DOI：
10.1007/s00795-019-00231-8
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发表时间：
2019-10-04
影响因子：
1.8
通讯作者：
Imaizumi, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Hiramatsu, Noriko;Yamamoto, Naoki;Imaizumi, Kazuyoshi
基于树突状细胞的免疫疗法使用患者自身的免疫细胞，可用于癌症治疗和过敏控制，例如与移植相关的自身免疫性疾病和排斥反应。然而，由于反复采血，这些治疗给患者带来了负担。我们使用细胞生物学分析的单核细胞与很少的突变从最小的血液采集基因组重组获得。接下来，我们利用商业载体和标准培养方法建立了人外周血单核细胞来源的诱导多能干细胞（iPSCs）。我们发现，当建立的iPSCs被诱导分化时，单核细胞表现出吞噬特性并表达CD14和CX3CR1。此外，在添加螨抗原后，生成的树突状细胞（dc）表达CCL17和高表达HLA-DR。综上所述，这些数据表明，单核细胞衍生的iPS细胞可以分化为单核细胞和dc。此外，这些细胞的使用可应用于树突状细胞治疗和单核细胞疾病的病理分析。
Dendritic cell-based immunotherapy, which uses a patient's own immune cells, can be used for cancer treatment and allergy control, such as autoimmune disease and rejection associated with transplantation. However, these treatments create a burden on patients due to repeated blood collection. We used cell biological analysis of monocytes with few mutations obtained from minimal blood collection for genome recombination. Next, we established human peripheral blood monocyte-derived induced pluripotent stem cells (iPSCs) using a commercial vector and standard culture method. We found that when established iPSCs were induced to differentiate, monocytes showed phagocytic properties and expressed CD14 and CX3CR1. Further, the generated dendritic cells (DCs) expressed CCL17 and highly expressed HLA-DR following the addition of the mite antigen. Taken together, these data show that monocyte-derived iPS cells can be used to differentiate into monocytes and DCs. In addition, the use of these cells can be applied to the pathological analysis of dendritic cell therapy and monocyte diseases.