An active transposable element, Herves, from the African malaria mosquito Anopheles gambiae
An active transposable element, Herves, from the African malaria mosquito Anopheles gambiae
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DOI：
10.1534/genetics.104.036145
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发表时间：
2005-02-01
期刊：
影响因子：
3.3
通讯作者：
Atkinson, PW
中科院分区：
文献类型：
--
作者：
Arensburger, P;Kim, YJ;Atkinson, PW
转座子已被证明是对多种植物、动物和微生物进行基因操作的宝贵工具。一些人表示，它们可以用来通过冈比亚按蚊的目标种群传播理想的基因，例如对疟原虫感染的抵抗力，从而使蚊子无法传播疟疾。为了实现这一点，转座子在最初进入基因组后必须保持可移动和完整。内源的活性II类转座元件。冈比亚并未被用作基因载体/驱动因子，因为尚未分离到任何一种。我们报道了从蚊子和蚊子中发现了一种活性的II类转座元件Heroes。冈比亚亚目。HEROES是一个独特的帽子分子亚家族的成员，其中包括来自柑橘属和葫芦科的Hopper-WE分子。HEROES在果蝇S2细胞和发育中的胚胎中的迁移率分析中表现出转位活性，并被用作黑腹果蝇胚系转化载体。Herves显示了与Hat元素、Hermes和Hobo相距遥远的目标位置偏好的变化。英雄也存在于一个。Arabiens和An.Merus的拷贝数与在An中发现的相似。冈比亚亚目。来自东非种群的初步数据与这种元素在蚊子中转位活跃的情况一致。
Transposable elements have proven to be invaluable tools for genetically manipulating a wide variety of plants, animals, and microbes. Some have suggested that they could be used to spread desirable genes, such as refractoriness to Plasmodium infection, through target populations of Anopheles gambiae, thereby disabling the mosquito's ability to transmit malaria. To achieve this, a transposon must remain mobile and intact after the initial introduction into the genome. Endogenous, active class II transposable elements from An. gambiae have not been exploited as gene vectors/drivers because none have been isolated. We report the discovery of an active class II transposable element, Heroes, from the mosquito An. gambiae. Heroes is a member of a distinct subfamily of hAT elements that includes the hopper-we element from Bactrocera dorsalis and B. cucurbitae. Heroes was transpositionally active in mobility assays performed in Drosophila melanogaster S2 cells and developing embryos and was used as a germ-line transformation vector in D. melanogaster. Herves displays an altered target-site preference from the distantly related hAT elements, Hermes and hobo. Heroes is also present in An. arabiensis and An. merus with copy numbers similar to that found in An. gambiae. Preliminary data from an East African population are consistent with the element being transpositionally active in mosquitoes.