Helicobacter pylori defines local immune response through interaction with dendritic cells
Helicobacter pylori defines local immune response through interaction with dendritic cells
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DOI：
10.1111/j.1574-695x.2010.00761.x
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发表时间：
2011-03-01
影响因子：
--
通讯作者：
Engstrand, Lars
中科院分区：
文献类型：
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作者：
Andres, Sonke;Schmidt, Heather-Marie A.;Engstrand, Lars
细菌病原体幽门螺杆菌定植于人胃和十二指肠粘膜，逃避宿主反应的清除，并与消化性溃疡疾病和胃腺癌的风险增加有关。树突状细胞（dc）是对幽门螺旋杆菌免疫反应的启动器。本研究的目的是调查幽门螺杆菌与dc之间的相互作用。为了确定幽门螺杆菌对单核细胞来源的dc成熟和活化的影响，我们研究了20株不同炎症背景的临床幽门螺杆菌菌株对腺癌胃上皮细胞的影响。根据感染部位的淋巴细胞和粒细胞浸润程度及细菌密度确定炎症背景。不同菌株对DC的成熟和活化作用不同。虽然成熟似乎与测试的任何毒力因子无关，但在将低炎症背景的菌株与中等或高炎症背景的菌株进行比较时，观察到促炎细胞因子白介素-12 （IL-12）、肿瘤坏死因子- α、IL-6和IL-1 β的平均水平显著增加。综上所述，DC对不同菌株的体外反应与个体宿主的临床结果相关，表明该细胞类型在调节菌株特异性幽门螺杆菌感染中起主要作用。
The bacterial pathogen Helicobacter pylori colonizes the human gastric and duodenal mucosa, evades clearance by the host response and is associated with peptic ulcer disease and an increased risk of gastric adenocarcinoma. Dendritic cells (DCs) are initiators of the immune response to H. pylori. The aim of the current study was to investigate the interaction between H. pylori with DCs. To determine the impact of H. pylori on the maturation and the activation of monocyte-derived DCs, the effect of 20 clinical H. pylori strains with different inflammatory backgrounds on adenocarcinoma gastric epithelial cells was investigated. The inflammatory background was defined according to the degree of lymphocyte and granulocyte infiltration and the bacterial density at the site of infection. DC maturation and activation varied after exposure to the different strains. While maturation appeared to be independent of any virulence factor tested, a significant increase in the average level of cytokine production was observed for the proinflammatory cytokines interleukin-12 (IL-12), tumour necrosis factor-alpha, IL-6 and IL-1 beta when comparing strains with low inflammatory backgrounds with those of the medium or high backgrounds. In conclusion, the DC response towards different strains in vitro was associated with the clinical outcome of the individual host, suggesting a major role of this cell type in modulating strain-specific H. pylori infection.