Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense
Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense
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DOI：
10.1016/j.hal.2007.04.004
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发表时间：
2007-11-01
期刊：
影响因子：
6.6
通讯作者：
Hong, Hua Sheng
中科院分区：
文献类型：
--
作者：
Su, Ran Qiang;Yang, Xiao Ru;Hong, Hua Sheng
细菌与有害藻华（HAB）物种之间的相互作用被认为是调节这些藻类种群动态和毒素产生的重要因素。从东海海域分离到一株对塔玛伦亚历山大藻有杀藻活性的海洋细菌SP48。通过聚合酶链反应扩增和序列分析对16S rDNA进行遗传鉴定。序列分析表明SP48最可能与γ -变形菌亚纲和假互生单胞菌属有亲缘关系。分离菌株SP48通过间接攻击表现出杀藻活性。除藻类衍生的DOM外，还需要其他有机营养素来合成未知的杀藻化合物，但p -葡萄糖苷酶不是杀藻活性的原因。细菌SP48所产的杀藻化合物具有耐热性，在酸性条件下不稳定，不受细菌生长所需的温度、盐度和初始pH的影响，都能很容易地合成。这是首次报道一种细菌可以杀死有毒的甲藻a . tamarense，这一发现增加了我们对细菌-藻类相互作用和细菌在赤潮藻华种群动态中的作用的认识。(C) 2007年Elsevier B.V.出版
Interactions between bacteria and harmful algal bloom (HAB) species have been acknowledged as an important factor regulating both the population dynamics and toxin production of these algae. A marine bacterium SP48 with algicidal activity to the toxic dinoflagellate, Alexandrium tamarense, was isolated from the Donghai Sea area, China. Genetic identification was achieved by polymerase chain reaction amplification and sequence analysis of 16S rDNA. Sequence analysis showed that the most probable affiliation of SP48 was to the gamma-proteobacteria subclass and the genus Pseudoalteromonas. Bacterial isolate SP48 showed algicidal activity through an indirect attack. Additional organic nutrients but not algal-derived DOM was necessary for the synthesis of unidentified algicidal compounds but P-glucosidase was not responsible for the algicidal activity. The algicidal compounds produced by bacterium SP48 were heat tolerant, unstable in acidic condition and could be easily synthesized regardless of variation in temperature, salinity or initial pH for bacterial growth. This is the first report of a bacterium algicidal to the toxic dinoflagellate A. tamarense and the findings increase our knowledge of bacterial-algal interactions and the role of bacteria during the population dynamics of HABs. (C) 2007 Published by Elsevier B.V.