Cynatratoside-C efficacy against theronts of Ichthyophthirius multifiliis, and toxicity tests on grass carp and mammal blood cells.
Cynatratoside-C efficacy against theronts of Ichthyophthirius multifiliis, and toxicity tests on grass carp and mammal blood cells.
复制标题
DOI：
10.3354/dao02925
复制
发表时间：
2015-11
影响因子：
1.4
通讯作者：
Yao-wu Fu;Qi-Zhong Zhang;De-hai Xu;Bin Wang;Jing-Han Liang;D. Lin
中科院分区：
文献类型：
--
作者：
Yao-wu Fu;Qi-Zhong Zhang;De-hai Xu;Bin Wang;Jing-Han Liang;D. Lin
多丝小瓜虫是一种纤毛虫原生寄生虫，其感染导致鱼类高死亡率，并在水产养殖中造成严重的经济损失。找到新的，有效的抗I抗体。在以前的研究中，通过生物测定指导的分级分离从白前中分离出白前苷-C。本文研究了白首乌苷-C的抗寄生虫活性，测定了白首乌苷-C对草鱼和哺乳动物血细胞的毒性，并评价了白首乌苷-C对寄生虫的保护作用。草鱼多丝状线虫感染。结果表明，0.25mgl ~（-1）的Cynatratoside-C在186.7 ± 5.8min内可全部杀灭草鱼，对草鱼感染的草鱼有较好的治疗效果，对草鱼的保护作用也较好。multifiliis侵扰。结果表明，白首乌苷C对草鱼的96 h半致死浓度（LC_（50））和4 h半有效浓度（EC_（50））分别为46.8和0.088 mg·l ~（-1）。溶血试验表明，白首乌苷C对家兔红细胞无细胞毒性。因此，Cynatratoside-C可能是一种安全有效的潜在杀寄生虫剂。多丝的
Infection by Ichthyophthirius multifiliis, a ciliated protozoan parasite, results in high fish mortality and causes severe economic losses in aquaculture. To find new, efficient anti-I. multifiliis agents, cynatratoside-C was isolated from Cynanchum atratum by bioassay-guided fractionation in a previous study. The present study investigated the anti-theront activity, determined the toxicity of cynatratoside-C to grass carp Ctenopharyngodon idellus and mammalian blood cells, and evaluated the protection of cynatratoside-C against I. multifiliis theront infection in grass carp. Results showed that all theronts were killed by 0.25 mg l-1 of cynatratoside-C in 186.7 ± 5.8 min. Cynatratoside-C at 0.25 mg l-1 was effective in treating infected grass carp and protecting naive fish from I. multifiliis infestation. The 96 h median lethal concentration (LC50) of cynatratoside-C to grass carp and 4 h median effective concentration (EC50) of cynatratoside-C to theront were 46.8 and 0.088 mg l-1, respectively. In addition, the hemolysis assay demonstrated that cynatratoside-C had no cytotoxicity to rabbit red blood cells. Therefore, cynatratoside-C could be a safe and effective potential parasiticide for controlling I. multifiliis.