Persisting expression of galanin in axotomized mamillary and septal neurons of adult rats labeled for c-Jun and NADPH-diaphorase
Persisting expression of galanin in axotomized mamillary and septal neurons of adult rats labeled for c-Jun and NADPH-diaphorase
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DOI：
10.1016/s0169-328x(97)00070-3
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发表时间：
1997-08-01
期刊：
影响因子：
--
通讯作者：
Herdegen, T
中科院分区：
文献类型：
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作者：
Brecht, S;Buschmann, T;Herdegen, T
在成年雄性大鼠单侧立体定向横断(a)乳丘束、(b)前脑内侧束、(c)穹穹膜束和(d)坐骨神经后，用免疫组织化学方法研究了神经肽甘丙氨酸的表达及其与c- jun转录因子和一氧化氮合酶（NOS）标记酶NADPH-diaphorase的共定位。这一手术导致(a)乳腺ncl神经元的轴突切开术。（MnM）， (b)黑质致密（SNC）和室旁ncl。(c)内侧隔（MS）和布罗卡垂直对角带（VDB）， (d)坐骨运动神经元和背根神经节（DRG）。在所有这些经轴突切除的神经元亚群中，c-Jun的表达在轴突切除后24 - 36小时出现，并在SNC中持续大量表达15天，在MnM、PF、MS、VBD、坐骨DRG和运动神经元中持续30-50天。甘丙氨酸在无梗MnM、MS和DRG中表达，而在SNC、PF和坐骨运动神经元中未见表达。甘丙肽免疫反应性（IR）在神经纤维横断后3 ~ 5天出现，在MnM、MS和DRGs中持续50天。胞质甘丙肽- ir几乎完全局限于细胞核c-Jun表达的神经元。此外，甘丙肽的表达与那些在MnM和DRG中表现出NADPH-diaphorase活性增加或在MS中表现出NADPH-diaphorase活性残留的神经元长期共定位。与甘丙氨酸非常相似，在SNC、PF或坐骨运动神经元中，nadph - diaphase不受axotcm的影响。我们的发现提示了丙氨酸和NOS （NADPH-diaphorase活性）表达的共同机制。由于甘丙氨酸启动子含有AP-1结合位点，c-Jun可能会在nos阳性中枢神经元中触发甘丙氨酸的持续诱导，这些神经元在肛切开术引起的损伤中存活下来。
In adult male rats, the expression of the neuropeptide galanin and its co-localization with the c-Jun transcription factor and the NADPH-diaphorase, the marker enzyme for the nitric oxide synthase (NOS), was investigated by immunohistochemistry in axotomized neurons following unilateral stereotaxic transection of the (a) mamillo-thalamic tract, (b) medial forebrain bundle, (c) fimbria fornix bundle and (d) sciatic nerve. This surgical procedure resulted in axotomy of neurons of (a) mamillary ncl. (MnM), (b) substantia nigra compacta (SNC) and paraventricular ncl. of thalamic (PF) neurons, (c) medial septum (MS) and vertical diagonal band of Broca (VDB), and (d) sciatic motoneurons and dorsal root ganglia (DRG). In all of these axotomized neuronal subpopulations, expression of c-Jun appeared between 24 and 36 h post-axotomy and persisted on substantial levels for 15 days in the SNC and for 30-50 days in the MnM, PF, MS, VBD, sciatic DRG and motoneurons. Expression of galanin was seen in axotomized MnM, MS and DRG, but not in SNC, PF and sciatic motoneurons. Galanin-immunoreactivity (IR) appeared between 3 and 5 days after nerve fiber transection and persisted up to 50 days in the MnM, MS and DRGs. The cytoplasmic galanin-IR was almost completely restricted to those neurons showing a nuclear c-Jun expression. Moreover, galanin expression showed a long-lasting co-localization with those neurons that exhibited an increased NADPH-diaphorase reactivity in the MnM and DRG or a residual NADPH-diaphorase reactivity in MS post-axotomy. Very similar to galanin, NADPH-diaphorase was not affected by axotcmy in the SNC, PF or sciatic motoneurons. Our findings suggest a common mechanism for galanin and NOS (NADPH-diaphorase activity) expression. Since the galanin promotor contains an AP-1 binding site, c-Jun might trigger the lasting induction of galanin in NOS-positive central neurons that survive the axotomy-evoked injury.