Mechanistic Considerations on Contact-Active Antimicrobial Surfaces with Controlled Functional Group Densities
Mechanistic Considerations on Contact-Active Antimicrobial Surfaces with Controlled Functional Group Densities
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DOI：
10.1002/mabi.201000398
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发表时间：
2011-04-08
影响因子：
4.6
通讯作者：
Tiller, Joerg C.
中科院分区：
文献类型：
--
作者：
Bieser, Arno M.;Tiller, Joerg C.
以对甲苯磺酰基纤维素为原料，分别用DBA和DDA进行转化，合成了一系列N-烷基-N，N-二甲基脱氧铵纤维素。具有这些水不溶性衍生物的表面涂层含有明确定义的季铵官能团和非活性疏水和亲水基团的密度。结果表明，这种表面对S.金黄色葡萄球菌需要DDA、BDA和疏水基团之间的微妙平衡。提出了一种机制，涉及从细菌细胞膜的阴离子磷脂的选择性粘附。这种所谓的磷脂海绵效应得到以下事实的支持：所有涂层都可以通过用SDS或带负电荷的磷脂处理而失活，但不能用中性磷脂处理。
A series of N-alkyl-N,N-dimethyldeoxyammonium celluloses is synthesized by converting tosyl celluloses with DBA and DDA, respectively. Surface coatings with these water-insoluble derivatives contain well-defined densities of quaternary ammonium functions and nonactive hydrophobic and hydrophilic groups. It is shown that the antimicrobial activity of such surfaces against S. aureus requires a delicate balance between DDA, BDA, and hydrophobic groups. A mechanism is proposed that involves the selective adhesion of anionic phospholipids from the bacterial cell membrane. This so-called phospholipid sponge effect is supported by the fact that all coatings could be deactivated by treatment with SDS or negatively charged phospholipids, but not with neutral phospholipids.