Impact of botanical oils on polyunsaturated fatty acid metabolism and leukotriene generation in mild asthmatics.
Impact of botanical oils on polyunsaturated fatty acid metabolism and leukotriene generation in mild asthmatics.
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DOI：
10.1186/1476-511x-12-141
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发表时间：
2013-10-02
影响因子：
4.5
通讯作者：
Chilton FH
中科院分区：
文献类型：
--
作者：
Arm JP;Boyce JA;Wang L;Chhay H;Zahid M;Patil V;Govindarajulu U;Ivester P;Weaver KL;Sergeant S;Israel E;Chilton FH
膳食中添加含有n-6和n-318碳链(18C)-多不饱和脂肪酸的植物油，如γ亚麻酸(Gla，18：3n-6)、硬脂酸(SDA，18：4n-3)和α亚麻酸(ALA，18：3n-3)，可以影响多不饱和脂肪酸的代谢，改变包括花生四烯酸(AA)代谢在内的炎症过程，改善炎症性疾病。在轻度哮喘患者的饮食中添加两种不同剂量的植物种子油，分别是含有SDA、ALA和GLA的两种植物种子油(波罗的油[Borago officinalis，BO]和植物油[Echium Platagineum；EO])。随后进行了三周的淘汰期。评估了这些饮食操作对几个生化终点的影响，包括体内多不饱和脂肪酸代谢和体外刺激白细胞产生白三烯。补充几种EO/BO组合可增加循环中20-22碳(20-22C)多不饱和脂肪酸，包括二十碳五烯酸(EPA)、二十二碳五烯酸(DPA)和二高马林酸(DGLA)，这些已被证明在不影响循环AA水平的情况下抑制AA代谢和炎症。BO/EO组合也抑制体外白三烯的生成，某些组合使刺激的嗜碱性粒细胞的半胱氨基白三烯的生成减少50%，使刺激的中性粒细胞的白三烯的生成减少35%。这项研究表明，饮食补充BO/EO改变了20-22C多不饱和脂肪酸水平，并以与减轻炎症相一致的方式减少了白三烯的产生。
Dietary supplementation with botanical oils that contain n-6 and n-3 eighteen carbon chain (18C)-PUFA such as γ linolenic acid (GLA, 18:3n-6), stearidonic acid (SDA, 18:4n-3) and α linolenic acid (ALA, 18:3n-3) have been shown to impact PUFA metabolism, alter inflammatory processes including arachidonic acid (AA) metabolism and improve inflammatory disorders. The diet of mild asthmatics patients was supplemented for three weeks with varying doses of two botanical seed oils (borage oil [Borago officinalis, BO] and echium seed oil [Echium plantagineum; EO]) that contain SDA, ALA and GLA. A three week wash out period followed. The impact of these dietary manipulations was evaluated for several biochemical endpoints, including in vivo PUFA metabolism and ex vivo leukotriene generation from stimulated leukocytes. Supplementation with several EO/BO combinations increased circulating 20–22 carbon (20–22C) PUFAs, including eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and dihommo-gammalinolenic acid (DGLA), which have been shown to inhibit AA metabolism and inflammation without impacting circulating AA levels. BO/EO combinations also inhibited ex vivo leukotriene generation with some combinations attenuating cysteinyl leukotriene generation in stimulated basophils by >50% and in stimulated neutrophils by >35%. This study shows that dietary supplementation with BO/EO alters 20–22C PUFA levels and attenuates leukotriene production in a manner consistent with a reduction in inflammation.