Detection of severe acute respiratory syndrome Coronavirus-2 in the tears of patients with Coronavirus disease 2019
Detection of severe acute respiratory syndrome Coronavirus-2 in the tears of patients with Coronavirus disease 2019
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DOI：
10.1038/s41433-020-0965-2
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发表时间：
2020-05-18
期刊：
影响因子：
3.9
通讯作者：
Safi, Sare
中科院分区：
文献类型：
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作者：
Karimi, Saeed;Arabi, Amir;Safi, Sare
目的由于关于2019冠状病毒病（COVID-19）患者眼部受累的报道很少，本研究旨在评估COVID-19患者泪液中是否存在严重急性呼吸综合征冠状病毒2（SARS-CoV-2）。方法在本前瞻性病例系列中，对43例重症COVID-19患者进行鼻咽和泪液采样。采用RT-PCR方法检测SARS-CoV-2。根据其病史记录眼部和全身体征和症状。结果患者平均年龄56 ± 13岁。发病时间1-7天，平均3.27天。41例患者（95.3%）在采样时出现发热。只有1例患者出现结膜炎。30例（69.8%）鼻咽样本和3例（7%）泪液样本对SARS-CoV-2呈阳性。结膜炎患者泪液检测结果为阳性。所有泪液RT-PCR结果阳性的患者鼻咽部RT-PCR结果均为阳性。结论该系列重症COVID-19患者的眼部表现罕见，但7%的患者结膜分泌物中存在病毒RNA。因此，即使没有眼部表现，也应考虑眼部传播的可能性。
Objectives Since there are few reports on the ocular involvement of coronavirus disease 2019 (COVID-19) patients, this study aimed to assess the presence of severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in the tears of patients with COVID-19. Methods In this prospective case series, nasopharyngeal and tear sampling of 43 patients with severe COVID-19 were performed. The quantitative reverse transcription polymerase chain reaction (RT-PCR) was conducted to detect SARS-CoV-2. Ocular and systemic signs and symptoms were recorded from their medical history. Results The mean age of patients was 56 +/- 13 years. The average disease time from initiation of symptoms was 3.27 days, range: 1-7 days. Forty-one patients (95.3%) had fever at the time of sampling. Only one patient had conjunctivitis. Thirty (69.8%) nasopharyngeal and three (7%) tear samples were positive for SARS-CoV-2. The result of tear sample was positive in the patient with conjunctivitis. All patients with positive tear RT-PCR results had positive nasopharyngeal RT-PCR results. Conclusions Ocular manifestation was rare in this series of severe COVID-19 patients, however, 7% of the patients had viral RNA in their conjunctival secretions. Therefore, possibility of ocular transmission should be considered even in the absence of ocular manifestations.