The protein kinase complement of the human genome
The protein kinase complement of the human genome
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DOI：
10.1126/science.1075762
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发表时间：
2002-12-06
期刊：
影响因子：
56.9
通讯作者：
Sudarsanam, S
中科院分区：
文献类型：
--
作者：
Manning, G;Whyte, DB;Sudarsanam, S
我们已经使用公开的和专有的基因组、互补DNA和表达序列标签(EST)序列对人类基因组的蛋白激酶互补(“Kinome”)进行了编目。这为全面分析正常和疾病状态下的蛋白质磷酸化提供了一个起点，并通过关注一个大的基因家族详细了解了人类基因组分析的现状。我们鉴定了518个可能的蛋白激酶基因，其中71个以前没有被报道或描述为蛋白激酶，我们扩展或纠正了另外56个蛋白激酶的蛋白质序列。新的基因包括经过充分研究的家族的成员以及以前未识别的家族的成员，其中一些在模式生物中保守。分类和与模式生物基因组的比较确定了同源群体，并突出了人类和其他谱系特有的扩展。我们还鉴定了106个蛋白激酶假基因。染色体图谱显示了几个小的激酶基因簇，并显示244个激酶映射到疾病基因或癌症扩增。
We have catalogued the protein kinase complement of the human genome (the "kinome") using public and proprietary genomic, complementary DNA, and expressed sequence tag (EST) sequences. This provides a starting point for comprehensive analysis of protein phosphorylation in normal and disease states, as well as a detailed view of the current state of human genome analysis through a focus on one large gene family. We identify 518 putative protein kinase genes, of which 71 have not previously been reported or described as kinases, and we extend or correct the protein sequences of 56 more kinases. New genes include members of well-studied families as well as previously unidentified families, some of which are conserved in model organisms. Classification and comparison with model organism kinomes identified orthologous groups and highlighted expansions specific to human and other lineages. We also identified 106 protein kinase pseudogenes. Chromosomal mapping revealed several small clusters of kinase genes and revealed that 244 kinases map to disease loci or cancer amplicons.