SOMATIC-CELL GENETICS AND GENE FAMILIES
SOMATIC-CELL GENETICS AND GENE FAMILIES
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DOI：
10.1126/science.6573776
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
RUDDLE, FH
中科院分区：
文献类型：
--
作者：
DEUSTACHIO, P;RUDDLE, FH
体细胞遗传分析在哺乳动物基因染色体定位中的应用已经得到了很好的确立。随着重组DNA探针和高效印迹技术的发展，允许单拷贝细胞基因的可视化，体细胞遗传学已经从全细胞表达的表型水平扩展到细胞基因组本身的水平。这种延伸已被证明是非常宝贵的基因不容易在体细胞杂交和多基因家族的研究，特别是假基因分散在不同的染色体在整个基因组中的基因的分析。
The utility of somatic cell genetic analysis for the chromosomal localization of genes in mammals is well established. With the development of recombinant DNA probes and efficient blotting techniques that allow visualization of single-copy cellular genes, somatic cell genetics has been extended from the level of phenotypes expressed by whole cells to the level of the cellular genome itself. This extension has proved invaluable for the analysis of genes not readily expressed in somatic cell hybrids and for the study of multigene families, especially pseudogenes dispersed in different chromosomes throughout the genome.