Biosynthesis of secondary metabolites in the rice blast fungus Magnaporthe grisea:: the role of hybrid PKS-NRPS in pathogenicity
Biosynthesis of secondary metabolites in the rice blast fungus Magnaporthe grisea:: the role of hybrid PKS-NRPS in pathogenicity
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DOI：
10.1016/j.mycres.2007.08.003
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发表时间：
2008-02-01
影响因子：
--
通讯作者：
Lebrun, Marc-Henri
中科院分区：
文献类型：
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作者：
Collemare, Jerome;Billard, Alexis;Lebrun, Marc-Henri
真菌次生代谢物是重要的农用化学和药理活性物质来源。在过去的十年里，人们开展了许多研究来表征真菌次生代谢物的生物合成途径。这一努力导致了新化合物、基因簇和关键酶的发现，并得到了最近公布的真菌基因组序列的极大支持。在这篇综述中，我们介绍了在水稻病原菌Maynaporthe grisea基因组以及其他真菌基因组中寻找与次生代谢有关的基因及其簇的结果。我们还对最近发现的编码聚酮合成酶和单个非核糖体肽合成酶模块(PKS-NRPS)的杂交基因进行了系统发育分析，因为与其他真菌相比，稻瘟病菌似乎富含这些酶。利用表达和功能研究的结果，我们讨论了这些PKS-NRP在稻瘟病菌无毒和致病中的作用。(C)2007年英国真菌学会。爱思唯尔有限公司出版。保留所有权利。
Fungal secondary metabolites are an important source of bioactive compounds for agrochemistry and pharmacology. Over the past decade, many studies have been undertaken to characterize the biosynthetic pathways of fungal secondary metabolites. This effort has led to the discovery of new compounds, gene clusters, and key enzymes, and has been greatly supported by the recent releases of fungal genome sequences. in this review, we present results from a search for genes involved in secondary metabolism and their clusters in the genome of the rice pathogen, Maynaporthe grisea, as well as in other fungal genomes. We have also performed a phylogenetic analysis of recently discovered genes encoding hybrids between a polyketide synthase and a single non-ribosomal peptide synthetase module (PKS-NRPS), as M. grisea seems rich in these enzymes compared with other fungi. Using results from expression and functional studies, we discuss the role of these PKS-NRPS in the avirulence and pathogenicity of M. grisea. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.