Germline Transgenesis in Ciona
Germline Transgenesis in Ciona
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海鞘种系转基因
DOI：
10.1007/978-981-10-7545-2_10
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发表时间：
2018
影响因子：
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通讯作者：
Sasakura Yasunori
中科院分区：
文献类型：
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作者：
Satoh Teruki;Iitsuka Takako;Shiraishi Akira;Hozumi Akiko;Satake Honoo;Sasakura Yasunori;Sasakura Yasunori
转基因是阐明生物现象背后的细胞和分子机制的不可或缺的方法。在Ciona中，已经产生了在其基因组中具有转基因插入的转基因系。转基因株系是有价值的，因为它们以非嵌合方式表达报告基因。这种非镶嵌方式使我们能够准确地观察组织和器官。转基因的插入可以破坏基因以产生突变体。插入突变是研究基因功能的极好方法。在玻璃海鞘中，gf前体基因的表达在雌性生殖系中经历表观遗传沉默。这种表观遗传沉默已被用来建立一种新的方法，敲低母亲的基因表达。基于种系转基因的遗传程序促进了对玻璃海鞘基因功能的研究。
Transgenesis is an indispensable method for elucidating the cellular and molecular mechanisms underlying biological phenomena. InCiona, transgenic lines that have a transgene insertion in their genomes have been created. The transgenic lines are valuable because they express reporter genes in a nonmosaic manner. This nonmosaic manner allows us to accurately observe tissues and organs. The insertions of transgenes can destroy genes to create mutants. The insertional mutagenesis is a splendid method for investigating functions of genes. InCiona intestinalis, expression of thegfpreporter gene is subjected to epigenetic silencing in the female germline. This epigenetic silencing has been used to establish a novel method for knocking down maternal expression of genes. The genetic procedures based on germline transgenesis facilitate studies for addressing gene functions inCiona.