Regeneration of functional sweat gland-like structures by transplanted differentiated bone marrow mesenchymal stem cells
Regeneration of functional sweat gland-like structures by transplanted differentiated bone marrow mesenchymal stem cells
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DOI：
10.1111/j.1524-475x.2009.00474.x
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发表时间：
2009-05-01
影响因子：
2.9
通讯作者：
Chen, Minliang
中科院分区：
文献类型：
--
作者：
Sheng, Zhiyong;Fu, Xiaobing;Chen, Minliang
深度烧伤后汗腺的再生一直是一个未解决的问题。由于缺乏排汗，大面积深度烧伤的幸存者在闷热的几个月里过着悲惨的生活。我们的想法是通过诱导骨髓间充质干细胞（MSC）在体外获得汗腺细胞的表型来解决这个问题。然后将这些细胞移植到五名患者深度烧伤愈合后切除脱水疤痕后产生的新鲜皮肤伤口中。术后2~12个月，碘淀粉出汗试验阳性，证明所有MSCs移植区域的排汗功能均恢复。组织学和生化观察证实间充质干细胞转化的汗腺细胞参与了功能性汗腺的恢复，并且从这些区域收集的汗液成分与从正常皮肤收集的汗液成分相似。这是首例成功移植间充质干细胞再生功能性汗腺的报道，这可能有助于解决未来大面积深度烧伤患者汗腺耗竭的问题。
Regeneration of sweat glands after deep burns has been an unsolved problem. Owing to lack of perspiration, survivors of an extensive deep burn injury are leading a miserable life in sultry months. It was our contemplation to solve this problem by inducing bone marrow mesenchymal stem cells (MSCs) to acquire the phenotype of sweat gland cells in vitro. Then these cells were transplanted into fresh skin wounds resulting from excision of anhydrotic scars after healing of deep burn injury in five patients. Two to 12 months after the procedure, it was proved that there was recovery of perspiration function in all the MSCs' transplanted areas, as evidenced by positive iodine-starch perspiration test. Histological and biochemical observation confirmed the involvement of MSCs transformed sweat gland cells in the recovery of functional sweat glands, and the components of sweat collected from these areas were similar to that collected from normal skin. This is the first report of successful transplantation of MSCs in regenerating functional sweat glands, which may help solve the problem of depletion of sweat glands in patients surviving extensive deep burns in the future.