High-resolution mapping of a major effect QTL from wild tomato Solanum habrochaites that influences water relations under root chilling.
High-resolution mapping of a major effect QTL from wild tomato Solanum habrochaites that influences water relations under root chilling.
复制标题
从野生番茄茄构象QTL的高分辨率映射，影响根部冷藏下的水关系。
DOI：
10.1007/s00122-015-2540-y
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
St Clair DA
中科院分区：
文献类型：
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作者：
Arms EM;Bloom AJ;St Clair DA
将一个控制盐芥快速启动水分胁迫耐受性的QTLstm 9高分辨率定位于9号染色体区域，该区域包含与非生物胁迫耐受性相关的基因。野生番茄（Solanum habrochaites）对干旱和低温等非生物胁迫表现出较强的耐受性。根冷（6 °C）通过阻碍水分从根到芽的移动而诱导快速发生的水分胁迫。S. habrochaites通过关闭气孔和维持芽膨压来响应这种变化，而栽培番茄（S.番茄）不能关闭气孔并枯萎。这种反应（根冷却下的芽膨压维持）是由9号染色体上的一个主要QTL（指定为stm 9）控制的，该QTL先前被精细定位到2.7 cM区域。对9号染色体的重组亚近等基因系进行标记选择，在两组重复实验（秋季和春季）中对根低温下的芽膨压维持进行表型分析，并将数据用于高分辨率定位QTL stm 9至0.32 cM区域。QTL定位揭示了一个单一的QTL，这是一致的春季和秋季数据集，表明基因或基因有助于根低温下的芽膨维持驻留在标记区间H9-T1673。在S.根据番茄参考基因组序列，该9号染色体区域是基因丰富的，并且含有与非生物胁迫耐受性相关的基因家族的代表。本文的在线版本（doi：10.1007/s 00122 -015-2540-y）包含补充材料，可供授权用户使用。
QTLstm9controlling rapid-onset water stress tolerance inS. habrochaiteswas high-resolution mapped to a chromosome 9 region that contains genes associated with abiotic stress tolerances. Wild tomato (Solanum habrochaites) exhibits tolerance to abiotic stresses, including drought and chilling. Root chilling (6 °C) induces rapid-onset water stress by impeding water movement from roots to shoots. S. habrochaites responds to such changes by closing stomata and maintaining shoot turgor, while cultivated tomato (S. lycopersicum) fails to close stomata and wilts. This response (shoot turgor maintenance under root chilling) is controlled by a major QTL (designated stm9) on chromosome 9, which was previously fine-mapped to a 2.7-cM region. Recombinant sub-near-isogenic lines for chromosome 9 were marker-selected, phenotyped for shoot turgor maintenance under root chilling in two sets of replicated experiments (Fall and Spring), and the data were used to high-resolution map QTL stm9 to a 0.32-cM region. QTL mapping revealed a single QTL that was coincident for both the Spring and Fall datasets, suggesting that the gene or genes contributing to shoot turgor maintenance under root chilling reside within the marker interval H9–T1673. In the S. lycopersicum reference genome sequence, this chromosome 9 region is gene-rich and contains representatives of gene families that have been associated with abiotic stress tolerance. The online version of this article (doi:10.1007/s00122-015-2540-y) contains supplementary material, which is available to authorized users.