Evidence for a nitrate-independent function of the nitrate sensor NRT1.1 in Arabidopsis thaliana
Evidence for a nitrate-independent function of the nitrate sensor NRT1.1 in Arabidopsis thaliana
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DOI：
10.1007/s10265-010-0385-7
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发表时间：
2011-05-01
影响因子：
2.8
通讯作者：
Noguchi, Ko
中科院分区：
文献类型：
--
作者：
Hachiya, Takushi;Mizokami, Yusuke;Noguchi, Ko
NRT1.1是一种可能的硝酸盐感受器，参与许多硝酸盐依赖的反应。另一方面，NRT1.1的硝酸盐非依赖性功能已被暗示，但明确的证据尚不清楚。我们发现，NRT1.1突变体对作为拟南芥唯一氮源的浓铵具有更强的耐受性。这种独特的表型在NLP7的突变体中没有观察到，NLP7被认为在硝酸盐依赖的信号通路中发挥作用。我们的实时聚合酶链式反应分析和文献调查的证据表明，与脂肪硫代葡萄糖苷生物合成途径相关的几个基因是通过NRT1.1的硝酸盐非依赖信号来调节的。综上所述，本研究强烈表明NRT1.1存在硝酸盐不依赖的功能。
NRT1.1 is a putative nitrate sensor and is involved in many nitrate-dependent responses. On the other hand, a nitrate-independent function of NRT1.1 has been implied, but the clear-cut evidence is unknown. We found that NRT1.1 mutants showed enhanced tolerance to concentrated ammonium as sole N source in Arabidopsis thaliana. This unique phenotype was not observed in mutants of NLP7, which has been suggested to play a role in the nitrate-dependent signaling pathway. Our real-time PCR analysis, and evidence from a literature survey revealed that several genes relevant to the aliphatic glucosinolate-biosynthetic pathway were regulated via a nitrate-independent signal from NRT1.1. When taken together, the present study strongly suggests the existence of a nitrate-independent function of NRT1.1.